Comparison of Effectiveness of ISSR and RAPD Markers in Genetic Characterization of Seized Marijuana (Cannabis Sativa L.) in Turkey

dc.contributor.authorKayış, Seyit Ali
dc.contributor.authorHakkı, Erdoğan Eşref
dc.contributor.authorPınarkara, Emine
dc.date.accessioned2020-03-26T17:47:33Z
dc.date.available2020-03-26T17:47:33Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis paper presents results from comparison of effectiveness of Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and ISSR and RAPD (ISSR-RAPD) markers together in characterization of Cannabis accessions. The plant material used was common accessions of psychoactive Cannabis samples (a total of 17 accessions), which were used in discriminating drug type Cannabis from hemp type Cannabis via ISSR and characterization of Cannabis samples via RAPD. Data were analyzed via cluster and principal coordinate analyses (PCoA). Discriminating powers of ISSR and RAPD markers on the seized Cannabis accessions were evaluated by utilising polymorphism information content, resolving power and marker index (MI). The PCoA of ISSR and ISSR-RAPD markers data produced similar results. Average resolving power and MI values of ISSR assay found to be slightly higher than those of RAPD assay. Consequently, ISSR markers would be a better choice compared to RAPD markers in characterization of Cannabis accessions.en_US
dc.description.sponsorshipScientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-Kariyer-104O547]; Scientific Research Coordination Center of Selcuk UniversitySelcuk University [BAP-07201006]en_US
dc.description.sponsorshipThe Scientific and Technical Research Council of Turkey (TUBITAK-Kariyer-104O547) and the Scientific Research Coordination Center of Selcuk University (BAP-07201006) are gratefully acknowledged for their supports. Authors thank Istanbul, Izmir, Adana, Malatya, and Trabzon forensic departments for providing research material.en_US
dc.identifier.citationKayış, S. A., Hakkı, E. E., Pınarkara, E., (2010). Comparison of Effectiveness of ISSR and RAPD Markers in Genetic Characterization of Seized Marijuana (Cannabis Sativa L.) in Turkey. African Journal of Agricultural Research, 5(21), 2925-2933.
dc.identifier.endpage2933en_US
dc.identifier.issn1991-637Xen_US
dc.identifier.issue21en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2925en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24721
dc.identifier.volume5en_US
dc.identifier.wosWOS:000285647100010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKayış, Seyit Ali
dc.institutionauthorHakkı, Erdoğan Eşref
dc.institutionauthorPınarkara, Emine
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Agricultural Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCannabis sativa L.en_US
dc.subjectindividualization of seized marijuanaen_US
dc.subjectinter simple sequence repeat (ISSR)en_US
dc.subjectrandomly amplified polymorphic DNA (RAPD)en_US
dc.subjectprincipal coordinate analysisen_US
dc.subjectcluster analysisen_US
dc.titleComparison of Effectiveness of ISSR and RAPD Markers in Genetic Characterization of Seized Marijuana (Cannabis Sativa L.) in Turkeyen_US
dc.typeArticleen_US

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