Autoinhibitory feedback control over photodynamic action

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dc.contributor.authorYurt, Mediha Nur Zafer.
dc.contributor.authorCakmak, Yusuf.
dc.contributor.authorTekin, Gulsum.
dc.contributor.authorKarakurt, Serdar.
dc.contributor.authorErbas-Cakmak, Sundus.
dc.date.accessioned2020-03-26T20:12:41Z
dc.date.available2020-03-26T20:12:41Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyokimya Bölümüen_US
dc.description.abstractIn biology, the activity of enzymes is usually regulated by feedback loops, which enables direct communication between enzymes and the state of the cell. In a similar manner, with the intention to have automated activity regulation, the therapeutic effect of a photosensitizer (BOD1) is shown to be reduced through a negative feedback loop initiated by the photosensitizer. Photodynamic action produces cytotoxic O-1(2) and this reactive oxygen species reacts with ascorbate, generating H2O2. Peroxide-mediated oxidation of the photosensitizer auxiliary group leads to the formation of inactive BOD2 from the parent photosensitizer. BOD1 is shown to accumulate in mitochondria, and cell viability is shown to decrease significantly with BOD1 compared to the loop end product, BOD2. Photoinduced enhancement of fluorescence indicates the formation of inactive BOD2 under cellular conditions, and enhanced fluorescence acts as a reporter for the activity of the photosensitizer. We present the first example of PDT autoinactivation, and such a feedback control mechanism would enable a decrease in post-therapy side effects.en_US
dc.description.sponsorshipBAGEP Award of the Science Academyen_US
dc.description.sponsorshipThis work was supported by the BAGEP Award of the Science Academy.en_US
dc.identifier.citationYurt, M. N. Z., Cakmak, Y., Tekin, G., Karakurt, S., Cakmak, S. E. (2019). Autoinhibitory Feedback Control over Photodynamic Action. ACS Omega, 4(7), 12293-12299.
dc.identifier.doi10.1021/acsomega.9b01410en_US
dc.identifier.endpage12299en_US
dc.identifier.issn2470-1343en_US
dc.identifier.issue7en_US
dc.identifier.pmid31460346en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage12293en_US
dc.identifier.urihttps://dx.doi.org/10.1021/acsomega.9b01410
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37521
dc.identifier.volume4en_US
dc.identifier.wosWOS:000482176800112en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTekin, Gulsum.
dc.institutionauthorKarakurt, Serdar.
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOCen_US
dc.relation.ispartofACS OMEGAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectphotodynamic therapy
dc.subjectphotosensitizer
dc.subjectH2O2
dc.titleAutoinhibitory feedback control over photodynamic actionen_US
dc.typeArticleen_US

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