Molecular markers to evaluate genetic diversity among Venturia inaequalis isolates obtained from apple plantations in Isparta Province

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dc.contributor.authorKaymak, Suat
dc.contributor.authorBoyraz, Nuh
dc.contributor.authorDaniels, Jon
dc.date.accessioned2020-03-26T19:25:10Z
dc.date.available2020-03-26T19:25:10Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTurkey, the third leading apple producer in world, produces approximately 2.6 million tons of apples per year. Venturia inaequalis, the causal agent of apple scab, is a major fungal disease among apples varieties. This study was conducted in accordance with the plant protection method for isolates collected from the apple production areas in Isparta Province. The purpose of this study was to determine genetic differences among V. inequalis isolates causing apple scab, and to discriminate the effects of varieties and geographical origins on genetic diversity. For this purpose, a total of 83 samples from the leaves and fruits of infected plants were collected to obtain isolates from apple scab. Of these, 67 isolates were obtained by isolating single conidia. Genetic differences and the relationship of the isolates were evaluated using random amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), simple sequence repeat (SSR), and sequence-related amplified polymorphism (SRAP) markers. The marker techniques SRAP and ISSR were used for the first time in this study to determine the genotype of the isolates of V. inaequalis. There was no relationship between apple varieties and geographic isolation or source of cultivar patterns. Variance analysis and molecular data of clustering from the isolates at district level revealed highly genetic similarity among populations. Additionally, SSR and SRAP markers were found to be more informative and consistent than other marker techniques. This study was the first report on population genetics of V. inaequalis identified with molecular markers in Turkey.en_US
dc.description.sponsorshipScientific Research Project Unit of General Directorate of Agricultural Research and Policy; Scientific Research Coordination Center of Selcuk University, TurkeySelcuk Universityen_US
dc.description.sponsorshipThis study was funded by the Scientific Research Project Unit of General Directorate of Agricultural Research and Policy. This research was supported as a PhD thesis by the Scientific Research Coordination Center of Selcuk University, Turkey.en_US
dc.identifier.doi10.3906/tar-1502-61en_US
dc.identifier.endpage498en_US
dc.identifier.issn1300-011Xen_US
dc.identifier.issn1303-6173en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage489en_US
dc.identifier.urihttps://dx.doi.org/10.3906/tar-1502-61
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33797
dc.identifier.volume40en_US
dc.identifier.wosWOS:000378090700002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF AGRICULTURE AND FORESTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAppleen_US
dc.subjectgenetic diversityen_US
dc.subjectISSRen_US
dc.subjectRAPDen_US
dc.subjectSRAPen_US
dc.subjectSSRen_US
dc.subjectVenturia inaequalisen_US
dc.titleMolecular markers to evaluate genetic diversity among Venturia inaequalis isolates obtained from apple plantations in Isparta Provinceen_US
dc.typeArticleen_US

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