Etanercept treatment in the endotoxin-induced uveitis of rats

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dc.contributor.authorAvunduk, MC
dc.contributor.authorAvunduk, AM
dc.contributor.authorOztekin, E
dc.contributor.authorBaltaci, AK
dc.contributor.authorOzyazgan, Y
dc.contributor.authorMogolkoc, R
dc.date.accessioned2020-03-26T16:48:36Z
dc.date.available2020-03-26T16:48:36Z
dc.date.issued2004
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study was conducted to investigate therapeutic value of a soluble tumor necrosis factor-alpha (TNF-alpha) receptor, etanercept, in a rat model of endotoxin-induced uveitis (EIU). Forty-two inbred male Lewis rats were divided into seven equal groups. 200 mug of Escherichia coli 055:1355 lipopolysaccharide, (LPS) was injected in one hind footpad of the Groups 2, 3, 4, 5, 6, and 7 rats. Group 5, 6, and 7 rats also received subcutaneous etanercept 24 hr prior to LPS injection at a dose of 0(.)4 mg kg(-1). Group 1 rats were used as controls. Eight, 24, and 48 hr after treatment clinical uvetis scores (miosis, iris hyperemia, and hypopyon) were assessed by a masked observer and the rats were euthanized. Neutrophil leukocytes, CD8 +, CD4 +, and CD45RO + cells in the anterior uveal tissue were counted either after hematoxylin-eosin or monoclonal antibody staining. TNF-alpha. levels were also measured in the aqueous humor samples by an ELISA method. Etanercept treatment significantly improved clinical uveitis scores at all examination points compared to the LPS injected animals. The improvement was almost complete expect for the miosis score, since no significant difference was detected between the controls and LPS + Etanercept treated animals at all examination points. Cell counts were also at significantly lower levels in LPS + Etanercept treated animals at all examination points, except for CD8 + and CD45RO + cell counts at 24 hr examination point. There was no significant difference between the controls and LPS + Etanercept treated animals at all examination points as with CD4 + and CD45RO + cell counts at 48 hr. Our data showed that etanercept had a definite effect on the treatment of EIU. Further studies should clarify its efficacy on clinical uveitis conditions. (C) 2004 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.exer.2004.06.001en_US
dc.identifier.endpage365en_US
dc.identifier.issn0014-4835en_US
dc.identifier.issn1096-0007en_US
dc.identifier.issue3en_US
dc.identifier.pmid15336498en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage357en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.exer.2004.06.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/19059
dc.identifier.volume79en_US
dc.identifier.wosWOS:000223973400008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDen_US
dc.relation.ispartofEXPERIMENTAL EYE RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectendotoxin-induced uveitisen_US
dc.subjectraten_US
dc.subjecttreatmenten_US
dc.subjectetanercepten_US
dc.titleEtanercept treatment in the endotoxin-induced uveitis of ratsen_US
dc.typeArticleen_US

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