Binding of actin to thioglycolic acid modified superparamagnetic nanoparticles for antibody conjugation

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dc.contributor.authorMaltas, Esra
dc.contributor.authorErtekin, Betul
dc.date.accessioned2020-03-26T19:01:06Z
dc.date.available2020-03-26T19:01:06Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThioglycolic acid modified superparamagnetic iron oxide nanoparticles (TG-APTS-SPION) were synthesized by using (3-aminopropyl) triethoxysilane (APTS) and thioglycolic acid (TG). Actin was immobilized on the nanoparticle surfaces. Binding amount of the actin (Act) on TG-APTS-SPIONs was determined by using a calibration curve equation that was drawn using fluorescence spectra at 280 and 342 nm of excitation and emission wavelengths. Anti-Actin (anti-Act) was interacted with the actin immobilized TG-APTS-SPIONs as primary antibody. Horse radish peroxidase (HRP) was also interacted with antibody conjugated nanoparticles as secondary antibody. The binding capacity of primary and secondary antibodies was also estimated by fluorescence spectroscopy. Scanning electron microscopy (SEM), Infrared spectroscopy (FTIR) and energy dispersive X-ray (EDX) analysis were also clarified binding of the protein and antibodies to the nanoparticles' surfaces. Western blot analysis was also done for actin conjuction with anti Act antibody to confirm binding of the antibody to the protein. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipResearch Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipWe would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work.en_US
dc.identifier.doi10.1016/j.ijbiomac.2014.10.006en_US
dc.identifier.endpage989en_US
dc.identifier.issn0141-8130en_US
dc.identifier.issn1879-0003en_US
dc.identifier.pmid25451750en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage984en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2014.10.006
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31889
dc.identifier.volume72en_US
dc.identifier.wosWOS:000347577900129en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntibodyen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectActinen_US
dc.subjectWestern blottingen_US
dc.subjectFluorescence spectroscopyen_US
dc.titleBinding of actin to thioglycolic acid modified superparamagnetic nanoparticles for antibody conjugationen_US
dc.typeArticleen_US

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