Knockout serum replacement is an efficient serum substitute for cryopreservation of human spermatozoa

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dc.contributor.authorTaher-Mofrad S.M.J.
dc.contributor.authorTopraggaleh T.R.
dc.contributor.authorZiarati N.
dc.contributor.authorBucak M.N.
dc.contributor.authorNouri M.
dc.contributor.authorSeifi S.
dc.contributor.authorEsmaeili V.
dc.date.accessioned2020-03-26T20:20:51Z
dc.date.available2020-03-26T20:20:51Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe freeze-thaw procedure causes irreversible structural and functional changes in human spermatozoa. In order to decrease the detrimental effects of cryopreservation and improve the quality of post-thawed spermatozoa, the constituents of the freezing solution attracted considerable attention. In this study, for the first time, we evaluated the efficacy of knockout serum replacement (KSR) as a substitute for human serum albumin (HSA) for cryopreservation of human spermatozoa. Twenty semen samples were collected from normozoospermic men and divided them into five equal groups. One of the aliquots was diluted with glycerol-based medium as a control group (CON). The other four aliquots were diluted with the sucrose solution containing 5% HSA (H5), 10% HSA (H10), 5% KSR (K5), and 10% KSR (K10). The diluted samples were frozen and preserved in liquid nitrogen. Post thawed sperm parameters including motion characteristics, viability, membrane integrity, mitochondrial activity, acrosome integrity and DNA intactness in all of the sucrose-based groups were comparable with glycerol-based medium. The replacement of HSA by 10% KSR in the freezing medium resulted in significantly higher post-thawed viability, acrosome integrity and DNA intactness compared with other sucrose-based groups. In conclusion, the addition of 10% KSR to the sucrose-based freezing solution improves the quality of post-thawed human spermatozoa and may have potential to develop chemically defined freezing medium. © 2020 Elsevier Inc.en_US
dc.description.sponsorshipRoyan Instituteen_US
dc.description.sponsorshipThis study was financially supported by the grant provided by Royan Institute . The authors gratefully thank the staff of sperm biology and clinical laboratories for providing facilities and kind assistance throughout the experiment.en_US
dc.identifier.doi10.1016/j.cryobiol.2020.01.013en_US
dc.identifier.issn0011-2240en_US
dc.identifier.pmid32004575en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2020.01.013
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38684
dc.identifier.wosWOS:000530033000028en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc.en_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCryopreservationen_US
dc.subjectHuman serum albuminen_US
dc.subjectHuman spermen_US
dc.subjectKnockout serum replacementen_US
dc.titleKnockout serum replacement is an efficient serum substitute for cryopreservation of human spermatozoaen_US
dc.typeArticleen_US

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