Genetic Variation in Turkish Honeybees Apis Mellifera Anatoliaca, A. m. Caucasica, A. m. Meda (Hymenoptera: Apidae) Inferred from RFLP Analysis of Three mtDNA Regions (16S rDNA-COİ-ND5)

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dc.contributor.authorÖzdil, Fulya
dc.contributor.authorAytekin, İbrahim
dc.contributor.authorİlhan, Fatma
dc.contributor.authorBoztepe, Saim
dc.date.accessioned2020-03-26T18:30:39Z
dc.date.available2020-03-26T18:30:39Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, the genetic structure of Turkish honey bee (Apis mellifera L.) populations, mainly obtained from the Central Anatolian region, were investigated at three different mitochondrial regions. A total of 165 worker bees were collected from 15 different populations in ten different locations. Portions of the mitochondrial 16S ribosomal RNA (16S rDNA), cytochrome C oxidase I (COI) and NADH dehydrogenase 5 (ND5) genes were amplified by PCR and then subjected to RFLP pattern analysis using 18 restriction enzymes (these having at least one recognition site in each region were used). Nucleotide polymorphisms were revealed using restriction enzymes Bsp1431, DraI and SspI in 16S rDNA and TaqI in the COI gene segment. The polymorphisms were subsequently confirmed by direct DNA sequencing with sequences thereafter deposited in Genbank. In this study, six novel composite genotypes (haplotypes) were found in Turkish honey bee populations. The most common haplotype, type 1, was found in 12 of the sampled populations and overall accounted for 85.5% of the samples. TCS spanning network of haplotypes revealed that type 1 was the basal haplotype. Genetic distance (D) values were found to be low (0.0-0.0112) within Turkish honey bee populations. The average haplotype diversity (h) within populations was 0.082. Molecular phylogenetic analysis revealed that Konya/Sizma, Antalya/Elmali and Konya/Selcuklu populations were the most distant from all the other Turkish honey bee populations surveyed.en_US
dc.description.sponsorshipScientific Research Projects (BAP) Coordinating Office of Selcuk University, TurkeySelcuk University [08401051]en_US
dc.description.sponsorshipThe authors thank Mehmet Ali Yildiz (Ankara University, Faculty of Agriculture) for providing insights and guidance during the various phases of this project. The project was supported by a grant from the Scientific Research Projects (BAP) Coordinating Office of Selcuk University, Turkey (Project No: 08401051).en_US
dc.identifier.citationÖzdil, F., Aytekin, İ., İlhan, F., Boztepe, S., (2012). Genetic Variation in Turkish Honeybees Apis Mellifera Anatoliaca, A. m. Caucasica, A. m. Meda (Hymenoptera: Apidae) Inferred from RFLP Analysis of Three mtDNA Regions (16S rDNA-COİ-ND5). European Journal of Entomology, 109(2), 161-167. Doi: 10.14411/eje.2012.021
dc.identifier.doi10.14411/eje.2012.021en_US
dc.identifier.endpage167en_US
dc.identifier.issn1802-8829en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage161en_US
dc.identifier.urihttps://dx.doi.org/10.14411/eje.2012.021
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28090
dc.identifier.volume109en_US
dc.identifier.wosWOS:000302587400004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖzdil, Fulya
dc.institutionauthorAytekin, İbrahim
dc.institutionauthorİlhan, Fatma
dc.institutionauthorBoztepe, Saim
dc.language.isoenen_US
dc.publisherCZECH ACAD SCI, INST ENTOMOLOGYen_US
dc.relation.ispartofEuropean Journal of Entomologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHymenopteraen_US
dc.subjectApidaeen_US
dc.subjectApis melliferaen_US
dc.subject16S rDNAen_US
dc.subjectCOIen_US
dc.subjectND5en_US
dc.subjectPCR-RFLPen_US
dc.subjectTurkeyen_US
dc.titleGenetic Variation in Turkish Honeybees Apis Mellifera Anatoliaca, A. m. Caucasica, A. m. Meda (Hymenoptera: Apidae) Inferred from RFLP Analysis of Three mtDNA Regions (16S rDNA-COİ-ND5)en_US
dc.typeArticleen_US

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