Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies

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dc.contributor.authorKaya, Murat
dc.contributor.authorMulercikas, Povilas
dc.contributor.authorSargın, İdris
dc.contributor.authorKazlauskaite, Sonata
dc.contributor.authorBaublys, Vykintas
dc.contributor.authorAkyüz, Bahar
dc.contributor.authorBulut, Esra
dc.date.accessioned2020-03-26T19:31:45Z
dc.date.available2020-03-26T19:31:45Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPhysicochemical characterization of new chitin isolates can provide valuable insights into designing of biomimetic materials. Chitin isolates with a definite three-dimensional (3D) structure can exhibit characteristics that distinguish them from other chitin specimens that are in form of powder or flakes without a definite and uniform shape. Herein, 3D chitin rings were produced from body segments of a diplopod (Archispirostreptus gigas) inhabiting tropical regions. This organism is cultured easily and can reach 38 cm in length, which makes it a suitable source for isolation of chitin. The chitin rings were characterized via TGA, FT-IR, SEM and XRD analyses. Enzymatic digestion test with chitinase demonstrated that chitin isolates had high purity (digestion rate: 97.4%). The source organism had high chitin content; 21.02 +/- 2.23% on dry weight. Interactions of the chitin rings with bovine serum albumin (BSA) protein were studied under different conditions (pH: 4.0-8.0, chitin amount: 6-14 mg, contact time: 30-360 min, protein concentration: 0.2-1 mg/mL). The highest BSA adsorption was observed at pH 5.0 at 20 degrees C. The adsorption equilibrium data exhibited a better fit to Langmuir adsorption and the pseudo-first order kinetic models. The findings presented here can be useful for further studies aiming to develop biocompatible and nontoxic biomaterials. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.msec.2016.06.046en_US
dc.identifier.endpage722en_US
dc.identifier.issn0928-4931en_US
dc.identifier.issn1873-0191en_US
dc.identifier.pmid27524072en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage716en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2016.06.046
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34147
dc.identifier.volume68en_US
dc.identifier.wosWOS:000382600000084en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subject3D chitinen_US
dc.subjectNanofibresen_US
dc.subjectNanoporousen_US
dc.subjectAlbuminen_US
dc.titleThree-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studiesen_US
dc.typeArticleen_US

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