The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers of Escherichia coli Strains by Multiplex PCR

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dc.contributor.authorOk, M.
dc.contributor.authorGuler, L.
dc.contributor.authorTurgut, K.
dc.contributor.authorOk, U.
dc.contributor.authorSen, I.
dc.contributor.authorGunduz, I. K.
dc.contributor.authorBirdane, M. F.
dc.date.accessioned2020-03-26T17:40:51Z
dc.date.available2020-03-26T17:40:51Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe purpose of this study was to determine aetiological agents of diarrhoea in neonatal calves and to investigate virulence gene markers of Escherichia coli strains isolated from calves by multiplex polymerase chain reaction (PCR). Eighty-two diarrhoeic calves and 18 healthy calves were used as subjects. Faeces were taken from the rectums of all the calves and were subjected to bacterial culture. Antigen enzyme-linked immunosorbent assay (ELISA) was performed to detect rotavirus, coronavirus and E. coli K99 in faeces of all the calves. A multiplex PCR was used to characterize E. coli strains in all the calves. Escherichia coli was isolated from 37 faeces samples, Enterococcus ssp. was isolated from 22 faeces samples and Salmonella was isolated from one faeces sample in diarrhoeic calves. Furthermore, only E. coli was isolated from all 18 faeces samples of healthy calves. Of the 37 E. coli isolated from diarrhoeic calves, K99 (18.9%), F41 (18.9%), heat-stable enterotoxin a (STa) (18.9%), Shiga toxin 1 (Stx1; 13.5%) and Shiga toxin 2 (Stx2; 5.4%) and intimin (8.1%) genes were identified by multiplex PCR. Of the 18 E. coli isolated from healthy calves, K99 (16.6%) and intimin (55.5%) genes were identified by PCR. A total of 15 rotavirus, 11 coronavirus and 11 E. coli K99 were detected in diarrhoeic calves by the antigen ELISA. As a result, this study shows that rotavirus, coronavirus, E. coli and Enterococcus ssp. were determined to play a role in the aetiology of diarrhoea in the neonatal calves. K99, F41, STa, Stx1 and Stx2 were found as the most common virulence gene markers of E. coli strains isolated from calves with diarrhoea. Multiplex PCR may be useful for characterization of E. coli isolated from calves.en_US
dc.description.sponsorshipSelcuk universitesi Scientific Research CoordinatorshipsSelcuk Universityen_US
dc.description.sponsorshipThis study has been supported by Selcuk universitesi Scientific Research Coordinatorships. We greatly appreciate their supports.en_US
dc.identifier.doi10.1111/j.1863-2378.2008.01156.xen_US
dc.identifier.endpage101en_US
dc.identifier.issn1863-1959en_US
dc.identifier.issn1863-2378en_US
dc.identifier.issue2en_US
dc.identifier.pmid18771515en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage94en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1863-2378.2008.01156.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23969
dc.identifier.volume56en_US
dc.identifier.wosWOS:000263046400007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofZOONOSES AND PUBLIC HEALTHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCalfen_US
dc.subjectdiarrhoeaen_US
dc.subjectgene marker of Een_US
dc.subjectcolien_US
dc.subjectPCRen_US
dc.titleThe Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers of Escherichia coli Strains by Multiplex PCRen_US
dc.typeArticleen_US

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