Investigating the effects of DNA demethylation in chemotherapy resistance by ChIP-on-Chip method

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dc.authorid0000-0002-7300-4075
dc.authorid0000-0003-1571-2986
dc.contributor.authorDemirel Kars, Meltem.
dc.contributor.authorKoygun, Gözde.
dc.date.accessioned2020-03-26T20:14:49Z
dc.date.available2020-03-26T20:14:49Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Teknoloji Fakültesi, Metalurji ve Malzeme Mühendisliği Bölümüen_US
dc.description.abstractOBJECTIVE This study aimed to determine the epigenetic basis of drug resistance mechanisms developed in MCF-7 breast cancer cell line that is resistant to an anticancer agent paclitaxel. Thus, we investigated the effects of the changes in DNA level on gene expression profile and proposed methods of inhibiting resistance by DNA modifications. METHODS We investigated the epigenetic basis of acquired drug resistance in whole genome by comparing chromosome immunoprecipitation in paclitaxel resistant MCF-7 (MCF-7/Pac) cells and in drug-sensitive (MCP-7/S) cells. For this analysis, DNA samples from both cell lines were immunoprecipitated and labeled with Cy3 and Cy5 fluorescent dyes. Hybridization and array scanning was performed with Agilent all-Genome Microarray platform that was designed to detect DNA methylation. Me obtained high-throughput information was analyzed with a bioinformatics analysis program. RESULTS The results showed that demethylation and epigenetic modulation of the DNA regions encoding 90 genes arc significant in the development of multiple drug resistance (MDR) in breast cancer. Some of these genes, ICAM4, COX6B2, ITGB8, SLC39A4, TUBB2C, COL6A1, DAPK1, RUNX3, SLC35F3, and MAP6, are important players in the development of drug resistance and cancer stem cells. CONCLUSION Studies on reversing multidrug resistance can be carried out by DNA modification or methylation of target genes regions on DNA. The results presented in this study may shed light on drug development studies to make DNA modifications.en_US
dc.description.sponsorshipSelcuk University BAP project [14401033]en_US
dc.description.sponsorshipSelcuk University BAP project (number 14401033).en_US
dc.identifier.citationKars, M. D., Koygun, G. (2019). Investigating the Effects of DNA Demethylation in Chemotherapy Resistance by Chip-On-Chip Method. Turkish Journal of Oncology, 34(2), 72-80.
dc.identifier.doi10.5505/tjo.2019.1866en_US
dc.identifier.endpage80en_US
dc.identifier.issn1300-7467en_US
dc.identifier.issue2en_US
dc.identifier.pmid#YOKen_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage72en_US
dc.identifier.urihttps://dx.doi.org/10.5505/tjo.2019.1866
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37941
dc.identifier.volume34en_US
dc.identifier.wosWOS:000469789600003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKoygun, Gözde.
dc.language.isoenen_US
dc.publisherKARE PUBLen_US
dc.relation.ispartofTURK ONKOLOJI DERGISI-TURKISH JOURNAL OF ONCOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDemethylationen_US
dc.subjectepigeneticsen_US
dc.subjectmultidrug resistanceen_US
dc.subjectMDRen_US
dc.subjectMCF-7en_US
dc.subjectpaclitaxelen_US
dc.titleInvestigating the effects of DNA demethylation in chemotherapy resistance by ChIP-on-Chip methoden_US
dc.typeArticleen_US

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