Comparison of Chondrocytes Produced from Adipose Tissue-Derived Stem Cells and Cartilage Tissue

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dc.contributor.authorMeric, Aysenur
dc.contributor.authorYenigun, Alper
dc.contributor.authorYenigun, Vildan Betul
dc.contributor.authorDogan, Remzi
dc.contributor.authorOzturan, Orhan
dc.date.accessioned2020-03-26T18:41:19Z
dc.date.available2020-03-26T18:41:19Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: Spontaneous cartilage regeneration is poor after a cartilage defect occurs by trauma, surgical, and other reasons. Importance of producing chondrocytes from stem cells and using tissues to repair a defect is getting popular. The aim of this study was to compare the effects of injectable cartilage produced by chondrocytes differentiated from adipose tissue-derived mesenchymal stem cells and chondrocyte cells isolated directly from cartilage tissue. Methods: Mesenchymal stem cells were isolated from rat adipose tissue and characterized by cell-surface markers. Then, they were differentiated to chondrocyte cells. The function of differentiated chondrocyte cells was compared with chondrocyte cells directly isolated from cartilage tissue in terms of collagen and glycosaminoglycan secretion. Then, both chondrocyte cell types were injected to rats' left ears in liquid and gel form, and histologic evaluation was done 3 weeks after the injection. Results: Adipose-derived stem cells were strongly positive for the CD44 and CD73 mesenchymal markers. Differentiated chondrocyte cells and chondrocyte cells directly isolated from cartilage tissue had relative collagen and glycosaminoglycan secretion results. However, histologic evaluations did not show any cartilage formation after both chondrocyte cell types were injected to rats. Conclusions: Strong CD44- and CD73-positive expression indicated that adipose-derived cells had the stem cell characters. Collagen and glycosaminoglycan secretion results demonstrated that adipose-derived stem cells were successfully differentiated to chondrocyte cells.en_US
dc.identifier.doi10.1097/SCS.0b013e3182902779en_US
dc.identifier.endpage833en_US
dc.identifier.issn1049-2275en_US
dc.identifier.issn1536-3732en_US
dc.identifier.issue3en_US
dc.identifier.pmid23714890en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage830en_US
dc.identifier.urihttps://dx.doi.org/10.1097/SCS.0b013e3182902779
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29313
dc.identifier.volume24en_US
dc.identifier.wosWOS:000330078200079en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLIPPINCOTT WILLIAMS & WILKINSen_US
dc.relation.ispartofJOURNAL OF CRANIOFACIAL SURGERYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectChondrogenesisen_US
dc.subjectcartilageen_US
dc.subjectchondrocytesen_US
dc.subjectadipose-derived stem cellsen_US
dc.subjectdifferentiationen_US
dc.titleComparison of Chondrocytes Produced from Adipose Tissue-Derived Stem Cells and Cartilage Tissueen_US
dc.typeArticleen_US

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