Yazar "Gürbilek, Mehmet" seçeneğine göre listele

Listeleniyor 1 - 20 / 41
  • Küçük Resim
    Öğe
    Blood and Fingernail Copper (Cu) and Magnesium (Mg) Levels of Children with Acute Lymphoblastic Leukemia
    (1998) Akkuş, İdris; Gürbilek, Mehmet; Çalışkan, Ümran; Kaptanoğlu, Bünyamin; Üner, Abdurrahman
    In this study, serum and fingernail Cu and Mg levels of 10 children with acute Iymphoblastic leukemia and 12 healthy children were determined. Copper and magnesium levels of the sera and supernatants were measured by atomic ab-sorption spectrophotometer. (Perkin-Elmer 400). Fingernail Cu and Mg levels of the patients were 8.67 ± 5.35 and 0.22 ± 0.12 \mumol/mg of nail respectively. The values for control subjects were 4.27 ± 2.32 and 0.14 ± 0.03 \mumol/mg of nail. Serum Cu and Mg levels of the patients were 525.1 ± 160.9 \mug/dl and 2.42 ± 0.54 mg/dl, and those of controls were 115.0 ± 70.7 \mug/dl and 2.20 ± 0.70 mg/dl respectively. The differences between serum Cu and fingernail Cu and Mg levels of the two groups were statistically significant which might be an index for the activity of the disease.
  • Küçük Resim
    Öğe
    Cardiac Troponin-I in the Serum of Infants of Diabetic Mothers
    (Greenwich Medical Media Ltd, 2003) Oran, Bülent; Çam, Lokman; Başpınar, Osman; Baysal, Tamer; Reisli, İsmail; Peru, Harun; Karaaslan, Sevim; Koç, Hasan; Gürbilek, Mehmet
    A transient form of hypertrophic cardiomyopathy has been previously described in infants of diabetic mothers. When it occurs, it is generally benign. The purpose of our study was to establish the extent of injury to the cardiomyocytes in symptomatic and asymptomatic patients with and without hypertrophic cardiomyopathy. Thus, we compared 35 consecutive patients to 20 healthy controls, establishing the significance, if any, of differences in cardiac troponin-I and creatine kinase, including its MB-fraction, and seeking to establish the value of these parameters in the diagnosis of cardiac injury. We also determined to levels of glucose and insulin in the serum, and took note of electrocardiographic and echocardiographic investigations. Values were determined at the 1st and 7th days after admission in the patients, while parameters were measured in the control group only on the first day. We found that the levels of cardiac troponin-I in the serum, known to be a marker for cardiac injury, were significantly elevated in symptomatic patients with life-threatening respiratory or haemodynamic distress. We speculate that transient ventricular hypertrophy is neither the cause nor the consequence of damage to the cardiomyocytes. It would be interesting, nonetheless, to determine the relationship, if any, between cardiomyocytic damage and clinical outcome.
  • Küçük Resim
    Öğe
    Comparative Effect of Oral Pulse and Intravenous Calcitriol Treatment in Hemodialysis Patients: The Effect on Serum Il-1 and Il-6 Levels and Bone Mineral Density
    (Karger, 2002) Türk, Süleyman; Akbulut, Mehmet; Yıldız, Alaattin; Gürbilek, Mehmet; Gönen, Said; Tombul, Zeki; Yeksan, Mehdi
    Introduction: Increased serum levels of bone-resorptive cytokines such as interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) have been implicated for changes in bone remodeling in hemodialysis patients. In this prospective randomized study, we aimed to compare the effect of oral and intravenous (IV) pulse calcitriol on serum levels of IL-1beta and IL-6. Patients and Methods: Twenty-eight hemodialysis patients were included and consecutively randomized to receive either oral (n = 14, M/F = 7/7, mean age 42 +/- 15 years) or IV pulse (n = 14, M/F = 6/8, mean age 38 +/- 14 years) calcitriol treatment. No difference was found between groups for age, sex distribution, primary renal disease, mean time on hemodialysis and baseline biochemical parameters including serum levels of IL-1beta and IL-6. Results: The percent fall of intact parathyroid hormone iPTH) was significantly less with oral compared to IV calcitriol between 0 and the 3rd month (32 +/- 21 vs. 56 +/- 28%, p = 0.03). However, the percent fall in iPTH at the 6th month of the therapy was not different in the oral group compared to the IV group (57 +/- 22 vs. 73 +/- 24%, p = 0.12). The increase in bone mineral densities was higher in the IV group than the oral group. Oral and IV calcitriol caused a significant fall in IL-1beta (p =0.02 and p= 0.03, respectively) and IL-6 levels (p = 0.02 and p < 0.001, respectively) at the 6th month of treatment. The percent fall in serum IL-6 levels at the 6th month was significantly greater in the IV compared to the oral group (61 18 vs. 36 +/- 33%, p = 0.04), while the percent changes in serum IL-1beta levels were similar. Conclusion: IV calcitriol therapy has a greater suppression of PTH at the 3rd month of the therapy. Despite no difference in serum PTH levels at the 6th month, IV therapy has a greater increase in bone mineral densities and a greater decrease in serum IL-6 levels. These findings suggest IV calcitriol treatment has a superior effect on bone remodeling by influencing the levels of bone-resorptive cytokines as compared to the oral therapy group, beyond its suppressive effect on iPTH.
  • Küçük Resim Yok
    Öğe
    Comparison of Effects of Somatostatine and Octreotide in the Treatment of Acute Pancreatitis
    (2001) Şahin, Mustafa; Avşar, Fatih Mehmet; Vatansev, Hüsamettin; Tekin, Şakir; Gürbilek, Mehmet; Karademir, Mehmet; Yılmaz, Osman
    Akut pankreatit ciddi bir hastalıktır ve tedavi seçeneklerinin yararları tartışılmaya devam etmektedir. Akut pankreatitte octreotid ve somatostatin 'in etkilerini araştırmaktadır. Çalışmaya 48 adet Spraque-Dawley raflar alındı. Raflar 4 gruba ayrıldı Her grupta ana biliopankreatit duktusbağlanarak akut pankreatit oluşturuldu. Grup I; kontrol grubu, Grup II; 35 mcg/kg/4 st somotostatin kuyruk veninden yapıldı, Grup III; 1.3 mcg/kg/8 st oktreotid subkutan yapıldı, Grup IV; her iki ürün birlikte aynı doz ve aralıklarla uygulandı. Ameliyat öncesi vepost-op 48.saatte serolojik, hematolojik ve gazometrik ölçümler için kan örnekleri alındı. Katlar çalışmanın 48. saatinde sakrifiye edildiler. Pankreas, karaciğer ve akciğerden doku örnekleri alındı.Toplam 15 rat öldü ve gruplararası mortalite oranları arasında anlamlı farklılık bulundu (p0.05). Ameliyat öncesi alınan kanda çalışılan serolojik, hematolojik ve gazometrik parametreler bütün gruplarda benzerdi. Post-op 48 saat sonra, 1. grupta 8, 2. grupta 5, 3. grupta 4 ve 4. grupta 5 parametre, preoperatif sonuçlara kıyasla daha kötü bulundu. Her grupta interstisyel ödem ve iltihabi hücre infiltrasyonu gözlendi., fakat sadece 1. grupta kanama alanları görüldü. Sonuç olarak, akut pankreatitin erken döneminde tedavi amacıyla kullanıldıkları zaman somatostatin ve oktreotid'in etkili olabilecekleri kanaatindeyiz.
  • Küçük Resim Yok
    Öğe
    The comparison of the nitric oxide levels before and after treatment in osteoporotic patients
    (BLACKWELL PUBLISHING, 2006) Aköz, Mehmet; Portakal, M.; Topçu, Cemile; Gürbilek, Mehmet; Köylü, O.
    [Abstract not Available]
  • Küçük Resim
    Öğe
    Correlation Between Tissue Lactate Levels and Electroencephalogram In Evaluating the Severity of Experimental Head Trauma
    (Lippincott Williams & Wilkins, 2002) Öğün, Cemile Öztin; Üstün, Mehmet Erkan; Duman, Ateş; Gürbilek, Mehmet; Genç, Bülent Oğuz
    Objective: The objective of this study was to develop an electroencephalographic grading scale for evaluating the severity of head trauma and assessing the correlation of this scale with brain tissue lactate concentrations. Design: Animal experiment. Setting: Animal research laboratory in a university hospital. Subjects: Thirty New Zealand rabbits were divided into three groups. Interventions: Rabbits were anesthetized, and bilateral frontoparietal craniectomy was performed. An electroencephalogram was recorded over the dura from both sides. After electroencephalographic recording, unilateral trauma was produced by using the weight drop method with a calculated force of 401) g(.)cm(-1) and 800 g(.)cm(-1) in group 2 (n = 10) and group 3 (n = 10), whereas in group I (n = 10) only craniectomy was performed. Electroencephalographic recording was repeated 60 mins after trauma or craniectomy, and cortical tissue samples were resected from both sides to evaluate tissue lactate concentrations in all three groups. Measurements and Main Results: Electroencephalographic recordings from both sides of the brain were evaluated together by using a 6-point scale (1 = best to 6 = worst) that was based on the presence or absence of electroencephalographic activity and the decrease in amplitude or frequency band of the electroencephalogram. Lactate was measured in resected tissue by using spectrophotometric enzymatic methods. One-way analysis of variance for repeated measures, Bonferroni-adjusted paired Student's t-test, Kruskal Wallis analysis of variance, Bonferroni-adjusted Mann-Whitney-U, and Spearman's correlation tests were used as appropriate for statistical analysis. We considered p < .05 to be significant. The difference in lactate concentrations was significant between the three groups (p < .05). Electroencephalographic grades were significantly different between the pretraumatic and posttraumatic recordings (p < .05) and between the three groups after craniectomy or trauma (p < .001). There was a positive high correlation between lactate concentrations and electroencephalographic grades. Conclusions. Tissue lactate concentrations and electroencephalograhic grades change with the severity of the trauma, and there is a strong positive correlation between tissue lactate concentrations and electroencephalographic grades.
  • Küçük Resim Yok
    Öğe
    Deneysel nekrotizan enterokolit modelinde ağız yolu ile verilen immünglobülin A'nın etkisi
    (2008) Aydoğdu, Bahattin; Yurtçu, Müslim; Akbulut, Seval; Gürbilek, Mehmet; Toy, Hatice; Günel, Engin
    Amaç: Deneysel/olarak oluşturulan nekrotizan enterokolit (NEK) modelinde, oral yoldan verilen immünglobülin A'nın sıçan barsağını koruyucu etkisini araştırmayı amaçladık. Gereç ve Yöntem: 40 yenidoğan sıçan 10'arlı gruplar halinde 4 gruba ayrıldı. Kontrol (K) grubu anne yanında bırakılırken, NEK (N), sham (S) ve tedavi (T) grubu anne sütü almadan, annesinden ayrı olarak 36C de ve % 60'lık nemde beslenme ve bakım sağlanmak üzere inkübatöre yerleştirildi. K grubundaki sıçanlar anne sütü ile beslendi. N grubundaki denekler doğar doğmaz, hiç anne sütü almadan annelerinin yanından ayrılarak formula mama ile beslendi. T grubundaki sıçanlara hiç anne sütü almadan formula mamaya ilaveten 600 mglkglgün 6 doz halinde saf oral IgA verildi. S grubundaki deneklere hiç anne sütü almadan mamaya ilaveten 0.1 mllkglgün immunoglobulin çözücüsü olan distile su verildi. Tüm gruplardaki sıçanlar 4. gün tartılarak sakrifiye edildi. Laparatomi sonrası ile-oçekal valvin 1 cm proksimalinden 2 cm'lik barsak segmenti histopatolojik inceleme için, geri kalan 10 cm'lik segment biyokimyasal inceleme için çıkartıldı. H&E boyama ile histopatolojik, ARC (Apoptosis Repressor With CARD) Ab-1 apopitozis kiti kullanılarak immunohistokimyasal, doku Myeloperooksidaz (MPO), Tümör nekrozis faktör alfa (TNF-a), ve İnterlökin altı (IL-6) bakılarak biyokimyasal değerlendirme yapıldı. Bulgular: N ve S gruplarında mortalite oranı T ve K gruplarına göre anlamlı olarak yüksek bulundu (P0.05). K grubunda istatistiksel olarak anlamlı% ağırlık artış olduğu tespit edilirken, diğer gruplarda ağırlık azalması tespit edildi (P0.05). Histopatolojik değerlendirme ve apopitozis dağılımına bakıldığında, T grubunun, N ve S grubundan anlamlı azalma olduğu görüldü (P0.05). Doku IL-6, TNF-a ve MPO seviyeleri incelendiğinde, T grubunun S ve N gruplarından anlamlı olarak düşük olduğu (P0.05), ayrıca T ile K grubu arasında anlamlı fark olmadığı tespit edildi (P0.05). Sonuç: Oral yolla verilen saf lgA'nın deneysel NEK modelinde, intestinal hasan azalttığı ve NEK' ı önlediği görüldü.
  • Küçük Resim
    Öğe
    Deneysel nekrotizan enterokolit modelinde ağızdan verilen immünglobülin G' nin etkisi
    (2008) Yurtçu, Müslim; Aydoğdu, Bahattin; Toy, Hatice; Gürbilek, Mehmet; Günel, Engin
    Amaç: Deneysel olarak oluşturulan nekrotizan enterokolit (NEK) modelinde, ağızdan verilen immünglobulin G’nin sıçan barsağında koruyucu etkisini araştırdık. Gereç ve Yöntem: 40 yenidoğan sıçan 10’arlı gruplar halinde 4 gruba ayrıldı. Kontrol (K) grubu anne yanında bırakılırken, NEK (N), sham (S) ve tedavi (T) grubu anne sütü almadan, annesinden ayrı olarak 36C’de ve % 60’lık nemde beslenme ve bakım sağlanmak üzere inkübatöre yerleştirildi. K grubundaki sıçanlar anne sütü ile beslendi. N grubundaki denekler doğar doğmaz, anne sütü almadan annelerinin ayrılarak formula mama ile beslendi. T grubundaki sıçanlara hiç anne sütü almadan formula mamaya ilaveten 1200 mg/kg/gün 6 doz olarak ağızdan saf Ig G verildi. S grubundaki deneklere hiç anne sütü almadan mamaya ek olarak 0.1 ml /kg/gün immunoglobülin çözücüsü olan distile su verildi. Tüm gruplardaki sıçanlar 4. gün tartılarak sakrifiye edildi. Laparatomiden sonra ileoçekal valvin 1 cm yukarısından 2 cm’lik bağırsak segmenti histopatolojik inceleme için, geri kalan 10 cm’lik kısmı biyokimyasal inceleme için çıkartıldı. H&E boyama ile histopatolojik, ARC (Apoptosis Repressor With CARD) Ab-1 apopitozis kiti kullanılarak immunohistokimyasal, Tümör nekrozis faktör alfa (TNF-?) ve İnterlökin altı (IL-6) bakılarak biyokimyasal inceleme yapıldı. Bulgular: N, S ve T gruplarında mortalite oranı K gruplarına göre anlamlı olarak yüksek bulundu (P0.038). K grubunda diğer gruplara göre anlamlı ağırlık artışı tespit edildi (P0.000). Histopatolojik değerlendirmede K grubunda villus hasarı, transmural nekroz ve apoptozisin, N, S ve T gruplarından anlamlı olarak az olduğu görüldü (P0.000). Apopitozisin de K grubunda, N, S ve T gruplarından anlamlı olarak az olduğu görüldü (P0.001). Doku IL-6 ve TNF-? seviyelerinin K grubunda, S, N ve T gruplarından anlamlı olarak az olduğu (P0.05), ayrıca N, S ve T grupları arasında anlamlı farklı olmadığı saptandı (P0.05). Sonuç: Ağızdan verilen saf IgG’nin deneysel NEK modelinde, biyokimyasal ve histopatolojik olarak intestinal yıkımı azaltmadığı ve NEK’i önlemediği gösterildi.
  • Küçük Resim
    Öğe
    Diyabetik Olmayan Akut Koroner Sendromlarda Erken Dönem Yeni Bir Risk Önbelirleyicisi Olarak "Geliş İnsülin Rezistans İndeksi (GİRİ)’nin" Değerlendirilmesi
    (2002) İçli, Abdullah; Gök, Hasan; Altunkeser, Bülent Behlül; Özdemir, Kurtuluş; Gürbilek, Mehmet; Gederet, Yavuz Turgut; Sökmen, Gülizar
    Amaç: İnsülin rezistansı birçok kardiyovasküler hastalık için bir risk göstergesidir. Ancak, bazılarının etyoloji ve prognozu üzerinde etkisi tam olarak açığa kavuşmamıştır. Bu çalışmamızda, insülin rezistansının pratik olarak belirlenmesinde yeni sunulmuş olan "Geliş İnsülin Rezistans İndeksi (GİRİ)'nin" diyabetik olmayan akut koroner sendromlarda erken dönem yeni bir risk önbelirleyicisi olup olmadığını araştırmayı amaçladık. Yöntem: Çalışmamıza akut miyokard infarktüsü (AMİ) (Grup I, yaş ortalaması 5812 yıl olan 72 hasta) ve kararsız angina pektoris (KAP), (Grup II, yaş ortalaması 5810 yıl olan 88 hasta) tanılarıyla koroner yoğun bakım ünitesine yatırılan ve koroner anjiyografi (KAG) yapılan 160 diyabetik olmayan hasta dahil edildi. Tüm hastalarda geliş insülin ve glukoz değerlerinin çarpımının, normal glukoz düzeyi (5mmol/L) ve insülin düzeyinin (5mU/L) çarpımına bölünmesiyle GİRİ hesaplandı. Hastaların ekokardiyografik olarak sol ventrikül ejeksiyon fraksiyonu (SVEF), duvar hareket skor indeksi (SVDHSİ) ve KAG'lerinden Gensini skor indeksi belirlenerek 30 gün boyunca major kardiyak olaylar (kalp yetersizliği, atriyal fibrilasyon, reinfarktüs, ciddi ventriküler aritmiler, atriyo-ventriküler blok, revaskülarizasyon gereksinimi ve mortalite) bakımından izlemleri yapıldı. Bulgular: Grup I'de GİRİ daha yüksek bulundu.(7.25.3'e karşın, 5.24.4, p0.01). Grup I'de GİRİ ile Gensini skoru ve SVDHSİ arasında pozitif (sıra ile; r0.41, p0.01; r0.48, p0.001), SVEF arasında ise negatif korelasyon (r-0.37, p0.001) tespit edildi. Ayrıca Grup I'de çok değişkenli regresyon analizinde, GİRİ ile Gensini skor indeksi (r0.23, p0.01) ve SVDHSİ (r0.43, p0.0001) arasındaki pozitif korelasyonun devam ettiği görüldü. Yine Grup I'de kalp yetersizliği (r0.42, p0,0001), atriyal fibrilasyon (r0.35, p0.002) ve reinfarktüs (r0.23, p0.04) arasında anlamlı korelasyon tespit edilirken, çok değişkenli regresyon analizinde de, kalp yetersizliği (r0.21, p0.007), atriyal fibrilasyon (r0.18, p0.01) ve reinfarktüs (r0.18, p0.01) ile anlamlı pozitif korelasyonun devam ettiği görüldü. Buna karşın Grup II'de GİRİ ile bu parametreler arasında anlamlı bir ilişki tespit edilmedi. Sonuç: Geliş İnsülin Rezistans İndeksi, AMİ ile gelen ve diyabetik olmayan hastalarda erken dönem yeni bir risk önbelirleyicisi olarak yüksek riskli alt grupların erken belirlenmesinde kullanılabilir. Aynı zamanda GİRİ, AMİ'lü hastalarda sol ventrikül disfonksiyonunun ve koroner arter hastalığının yaygınlığını belirleyen bağımsız bir risk faktörü olarak, pratik olarak hesaplanabilen ve geniş ölçekli klinik çalışmalarda kolaylıkla kullanılabilir bir parametredir.
  • Küçük Resim Yok
    Öğe
    The effect of allopurinol and acetylsalicylate on ischemia-reperfusion related injury of liver
    (1999) Aköz, Mehmet; Dilsiz, Alaeddin; Gürbilek, Mehmet; Kaymakçı, Aytekin; Ay, Mahmut; Gültekin, Fatih
    Dokularda iskemi sırasında meydana gelen hasarın reperfüzyon sırasında arttığı, bu artışın da biyomembranlarda oksijen kaynaklı serbest radikallerin yol açtığı lipid peroksidasyonundan kaynaklandığı kabul edilir. Serbest radikaller çeşitli kaynaklardan oluşurlar. Bu kaynaklardan ikisi; ksantin oksidaz sistemi ve araşidonik asit yoludur. Bu çalışmanın amacı, bu sistemlerin lipid peroksidasyonu ve serbest oksijen radikalleri üretimi üzerine olan etkisini karaciğer iskemi-reperfüzyonu rat modeliyle değerlendirmektir. Bu maksat için 28 Wistar albino rat 5 gruba ayrıldı. Kontrol grubundaki ratlar üzerinde operasyon yapılmadı. İkinci grupta hepatik arterin sağ üst kolu ve portal ven 90 dakika süre ile klempe edilmek suretiyle iskemi oluşturuldu. Üçüncü grupta 90 dakikalık iskemi periyodu sonunda klampları açmak suretiyle 30 dakika süre ile reperfüzyon sağlandı. Aynı iskemi ve reperfüzyon işlemleri, allopurinol verilen dördüncü grup ve asetilsalisilat verilen beşinci grup ratlar üzerine uygulandı. Allopurinol ve asetilsalisilat oral yoldan işlemlerden önce üç gün süre ile verildi. Bütün gruplarda tiobarbitürat metodu kullanarak malondialdehit-tiobarbiturik asid (MDA-TBA) seviyelerini ölçmek suretiyle lipid peroksidasyonu değerlendirildi. İskeminin lipid peroksidasyonunu arttırdığı, bu artışın reperfüzyon sırasında fazlalaştığı görüldü. Lipid peroksidasyonu reperfüzyon grubu ile karşılaştırıldığında allopurinol grubunda anlamlı bir şekilde azaldığı (p0.001) görülürken asetilsalisilat grubunda değişmediği gözlendi. Bulgularımız, iskemi-reperfüzyon ile ilgili karaciğer hasarı üzerinde ksantin oksidaz sisteminin araşidonik asit yolundan daha etkili olduğunu, allopurinolün iskemi-reperfüzyon hasarı üzerine koruyucu bir etkiye sahip olduğunu göstermektedir.
  • Küçük Resim
    Öğe
    Effect of Deferoxamine on Na(+)K(+)ATPase Activity After Cerebral Ischemia in Rabbits
    (Riyadh Armed Forces Hospital, 2006) Gürbilek, Mehmet; Topçu, Cemile; Aköz, Mehmet; Barışkaner, Hülagü; Üstün, Mehmet E.; Köylü, Öznur
    Objective: To study the effects of deferoxamine on tissue sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) activity on cerebral ischemia in rabbits. Methods: We cared for the animals in the Pharmacology Department of the Medical School of Selcuk University in 2004. We used 30, New Zealand, 7-day-old male rabbits in the experiment. We anesthetized all the animals with xylazine hydrochloric acid and ketamine. We divided the rabbits equally into 3 groups. In group 1 (n=10) (sham group), we observed baseline levels, and did not apply ischemia. In group 2 (n=10) (untreated group) we produced cerebral ischemia by clamping the bilateral common carotid arteries for 60 minutes, and in group 3 (n=10), we administered deferoxamine (DFO) 50 mg/kg intravenously immediately after opening the clamps. Results: The Na+-K(+)ATPase activity increased after DFO treatment (p=0.045). Conclusion: We conclude that Na+-K(+)ATPase activity in cortical brain tissue was higher in DFO-treated rabbits compared with untreated animals after ischemia.
  • Küçük Resim Yok
    Öğe
    Effect of deferoxamine on Na+K+ATPase activity after cerebral ischemia in rabbits
    (2006) Gürbilek, Mehmet; Topcu, Cemile; Aköz, Mehmet; Barışkaner, Hülagü; Üstün, Mehmet E.; Köylü, Öznur
    Objective: To study the effects of deferoxamine on tissue sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) activity on cerebral ischemia in rabbits. Methods: We cared for the animals in the Pharmacology Department of the Medical School of Selcuk University in 2004. We used 30, New Zealand, 7-day-old male rabbits in the experiment. We anesthetized all the animals with xylazine hydrochloric acid and ketamine. We divided the rabbits equally into 3 groups. In group 1 (n=10) (sham group), we observed baseline levels, and did not apply ischemia. In group 2 (n=10) (untreated group) we produced cerebral ischemia by clamping the bilateral common carotid arteries for 60 minutes, and in group 3 (n=10), we administered deferoxamine (DFO) 50 mg/kg intravenously immediately after opening the clamps. Results: The Na +-K+ATPase activity increased after DFO treatment (p=0.045). Conclusion: We conclude that Na+-K+ATPase activity in cortical brain tissue was higher in DFO-treated rabbits compared with untreated animals after ischemia.
  • Küçük Resim
    Öğe
    Effect of Oral Glucose Loading on Serum Gastrin Level in Pregnant and Non-Pregnant Women
    (PABST SCIENCE PUBLISHERS, 2001) Gültekin, Fatih; Gürbilek, Mehmet; Vatansev, Hüsamettin; Yaşar, Sulhattin; Aköz, Mehmet; Öztürk, Mustafa
    Aim. To evaluate the relationship between the changes in gastrin and insulin serum concentrations after oral glucose loading in pregnant and non-pregnant women. Methods. Thirty women, 12 pregnant and 18 non-pregnant, with normal fasting glucose values were included in the study. Serum concentrations of gastrin, glucose, insulin, and glucagon were analyzed at 0 (t1), 30 (t2) and 60 (t3) minutes after 75 g oral glucose loading. Gastrin, insulin, and glucagon levels were determined by means of radioimmunoassay kits. Results. Serum gastrin concentration in pregnant women increased insignificantly (gastrin median values 57.91, 70.62, and 68.70 for t1, t2, and t3, respectively; Friedman's test, p=0.264). In non-pregnant women gastrin levels insignificantly increased from t1 to t2, but reduced significantly from t2 to t3 (gastrin median values 62.91, 86.92, and 62.25 for t1, t2 and t3, respectively; Bonferroni adjusted Wilcoxon test, p=0.002). Unlike in pregnant women, the changes in gastrin release in non-pregnant women were associated with changes in blood glucose concentrations at t2 and t3, which were induced by oral glucose loading. Glucose median values were 7.48 and 6.43 for t2 and t3, respectively. The insulin release due to the oral glucose loading markedly increased at t2 and t3 (Friedman's test, p<0.001), whereas glucagon release decreased irrespective of pregnancy. Conclusion. Changes in blood glucose concentrations induced by oral glucose loading could influence gastrin release, especially in non-pregnant women. Changes in insulin and glucagon levels induced by oral glucose loading, particularly after 60 minutes, could not be associated with changes in gastrin release.
  • Küçük Resim Yok
    Öğe
    Effect of oral immunoglobulin A in experimental necrotisan enterocolitis model
    (2008) Aydoğdu, Bülent; Yurtçu, Müslim; Akbulut, Seval; Gürbilek, Mehmet; Toy, Hatice; Günel, Engin
    Aim: Investigation of the protective effect of oral immunglobulin(Ig) A on rat intestinum in experimental necrotising enterocolitis (NEC) model. Materials and methods: 40 newborn rats were divided into 4 groups each containing 10 rats. While control (C) group was fed by breast, the rats in necrotisinge enterocolitis (N), sham (S), and treatment (T) groups were settled into incubators at 36°C and 60 % humidity and fed, but not by breast. The rats in C group were fed by breast. The rats in N group were fed with Formula as soon as they were born. The rats in T group were fed with Formula and 600 mg/kg/day oral Ig A with 4-hour intervals. The rats in S group were fed with Formula and 0.1 ml/kg/day distilled water which is solvent of Ig. The rats in all groups were weighed and sacrified on fourth day. 2 cm intestinal segment from proximal of ileocaecal valve was used for histopathologic examination, another 10 cm intestinal segment for biochemical examination. After laparotomy, H&E was used for histopathological examination and apoptosis repressor with card Ab-l citt for immunohistochemical examination. Biochemical parameters such as myeloperoxidase (MPO), TNF-?, and IL-6 were evaluated. Results: The rate of mortality in N and S groups was significantly higher than T and C groups (P<0.05). Significant weight increase was identified in C group (P<0.05) There was significant decrease in T group in comparison of histopathologic values and apoptosis according to N and S groups (P<0.05). T group was significantly different in comparison of IL-6, TNF-?, and MPO according to S and N groups (P<0.05). There was no significant difference between T and C groups (P>0.05). Conclusion: Pure IgA given orally was identified to decrease intestinal damage and to prevent NEC in experimental NEC model.
  • Küçük Resim
    Öğe
    Effects of Deferoxamine on Tissue Lactate and Malondialdehyde Levels in Cerebral Ischemia
    (Prous Science, Sa , 2003) Barışkaner, Hülagü; Üstün, M. E.; Ak, A.; Yosunkaya, Alper; Doğan, N.; Gürbilek, Mehmet
    In the present study, the effects of deferoxamine on tissue lactate and malondialdehyde (MDA) levels after cerebral ischemia at rabbits was studied. Rabbits were divided equally, into three groups: group 1: sham-operated group; group 2: cerebral ischemia produced by clamping bilateral common carotid arteries for 60 min: and group 3: deferoxamine 100 mg/kg i.v administered within 5 min after opening the clamps. EEG recordings were obtained in all groups before clamping and in group 2 and 3 60 min after clamping and 60 min after opening the clamps. One hour after opening the clamps and taking EEG recordings, brain cortices were resected and the concentrations of lactate and MDA were determined using the spectrophotometric enzymatic and thiobarbituric acid methods, respectively. There were significant differences between group I and the other groups in MDA and lactate levels (p < 0.05). There were no significant differences in lactate levels between groups 2 and 3. Preischemic EEG grades were the same at till groups. Preischemic (aid postischemic EEG values were significantly different (p < 0.05), but there were no significant differences between postischemic EEG grades in groups 2 and 3. There was also a correlation between postischemic EEG grades and lactate levels, but no correlation between postischemic EEG grades and MDA levels. These results demonstrate that cerebral ischemia leads to an increase in brain tissue lactate and MDA levels and deferoxamine suppresses the increase of MDA, but not lactate. Deferoxamine treatment caused no significant EEG changes. EEG grades correlated well with lactate levels.
  • Küçük Resim
    Öğe
    Effects of Deferoxamine on Tissue Superoxide Dismutase and Glutathione Peroxidase Levels in Experimental Head Trauma
    (LIPPINCOTT WILLIAMS & WILKINS, 2001) Üstün, Mehmet Erkan; Duman, Ateş; Öğün, Cemile Öztin; Sümer, Fatih; Gürbilek, Mehmet
    Background: This study aims to evaluate the effects of deferoxamine on tissue superoxide dismutase (SOD) and glutathione peroxidase (GPx) brain levels after head trauma. Methods: Thirty rabbits were divided equally into three groups: group 1 was the sham-operated group, group 2 suffered head trauma (no treatment was given), and group 3 received deferoxamine 50 mg/kg after the trauma. Head trauma was applied unilaterally. One hour after trauma, brain cortices were resected and SOD and GPx levels were determined. One-way analysis of variance and Tukey-HSD tests were used for analysis. Significance was defined asp < 0.05. Results: Baseline SOD levels are preserved in the traumatized side of the deferoxamine-treated group. Although GPx level of the traumatized side of the deferoxamine-treated group decreased significantly, the decrease was significantly less than the nontreated group. Conclusion: Trauma leads to a decrease in brain tissue SOD and GPx levels, Deferoxamine suppresses this decrease completely in SOD level and partially in GPx level when given after trauma.
  • Küçük Resim
    Öğe
    Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels After Cerebral Ischemia
    (Karger, 2003) Barışkaner, Hülagü; Üstün, M. E.; Ak, A.; Yosunkaya, Alper; Ulusoy, H. B.; Gürbilek, Mehmet
    In the present study, the effects of magnesium sulfate on tissue lactate and malondialdehyde (MIDA) levels after cerebral ischemia in rabbits were studied. The rabbits were divided equally into three groups. Group 1 (n = 8) was the sham-operated control group, in group 2 (n = 8) only cerebral ischemia was induced by clamping bilaterally the common carotid arteries for 60 min, and in group 3 (n = 8) magnesium sulfate was administered at a dose of 100 mg/kg im. within 5 min after opening the clamps. In group 1 EEG recordings were obtained immediately and 60 and 120 min after craniectomy. In groups 2 and 3 EEG recordings were obtained immediately after craniectomy but before clamping and 60 min after clamping. One hour after opening the clamps and taking EEG recordings, brain cortices were resected, and the concentrations of lactate and MIDA were determined using spectrophotometric/enzymatic and thiobarbituric acid methods, respectively. In all groups, there were significant differences between MDA and lactate levels (p < 0.05). There were no significant differences in lactate levels between groups 2 and 3 (p > 0.05), and also the preischemic EEG grades were the same in all groups. Preischemic and postischemic EEG values were significantly different (p < 0.05), and there were also significant differences between postischemic EEG grades in groups 2 and 3 (p < 0.05). There was a correlation between postischemic EEG grades and MIDA and lactate levels. These results demonstrate that cerebral ischemia-reperfusion injury leads to an increase in brain tissue lactate and MIDA levels, that magnesium sulfate suppresses the increase of MDA and lactate concentrations, and that magnesium sulfate, treatment improves the EEG changes. The EEG grades correlated well with MIDA and lactate levels.
  • Küçük Resim
    Öğe
    Effects of Magnesium Sulfate on Tissue Lactate and Malondialdehyde Levels in Experimental Head Trauma
    (SPRINGER-VERLAG, 2001) Üstün, Mehmet Erkan; Gürbilek, Mehmet; Ak, Ahmet; Vatansev, Hüsamettin; Duman, Ateş
    Objective: To determine the effects of magnesium sulfate (MgSO4) on tissue lactate and malondialdehyde (MDA) levels in rabbit brain after experimental head trauma. Design: Prospective, randomized trial. Subjects: Thirty New Zealand rabbits. Interventions: Group 1 (n = 10) was the sham operated group. Group 2 (n = 10) (untreated group) and group 3 (n = 10) received head trauma with the weight drop method. MgSO4 was administered 100 mg/kg (15%) i.v. immediately after the head trauma to group 3. Trauma was applied to one side. The non-contused side was named as "a" and the contused side as "b". Measurements: One hour after trauma, brain cortices were resected and the concentrations of lactate and MDA were determined using the spectrophotometric enzymatic and thiobarbituric acid methods. One-way ANOVA and Tukey's HSD tests were used for the evaluation of the results. P < 0.05 was considered as significant. Pearson's correlation test was used between lactate and MDA levels (P < 0.001). Results: There were significant differences between MDA and lactate levels of group 1 and all other groups; non-contused (a) and contused (b) sides of groups 2 and 3; groups 2b-3a, 2b-3b (P < 0.05). The difference in MDA levels was significant between groups 2a-3b (P < 0.05). Correlation between lactate and MDA was very good in group 1, and excellent in groups 2a, 2b, 3a, and 3b. Conclusions: These results demonstrate that head trauma leads to an increase in brain tissue lactate and MDA levels, and MgSO4 suppresses the rise in contused tissue when given after head trauma.
  • Küçük Resim
    Öğe
    Effects of Oral L-Glutamine, Insulin and Laxative on Bacterial Translocation in Acute Pancreatitis
    (2001) Avşar, Fatih Mehmet; Özel, İ. Hakan; Baykan, Mahmut; Saygun, Oral; Şahin, Mustafa; Hengirmen, Süleyman; Ertuğrul, Ömür; Gürbilek, Mehmet; Aydın, S. Neşe
    Aim: To investigate the effects of oral L-glutamine, insulin and laxative on the bacterial population of intestinal lumen and on bacterial translocation in acute pancreatitis. Materials and Methods: Forty Sprague-Dawley adult male rats were divided into 4 groups. Experimental pancreatitis was induced by ligating the main biliopancreatic duct. All groups were given the standard rat diet and tap water. In addition. Group II was given. 15 mg/kg/day of L-glutamine via a catheter enterally, Group III was given 3 ml of laxative via a catheter enterally, and Group IV was given 3 IU/kg/day of NPH insulin via a catheter enterally. The rats were sacrificed 96 hours after the induction of pancreatitis. Blood samples for biochemical analyses and blood culture, and culture samples from mesenteric lymph nodes, liver and spleen and from cecal content were taken. Aerobic and anaerobic cultures were prepared. Findings: Amylase levels in all the groups after the procedure increased significantly. Bacterial translocation was observed in 6 rats in the control group, in 5 rats in the insulin group, in 3 rats in the laxative group and in 2 rats in the L-glutamine group. A significant decrease in the number of luminal bacteria was observed in the laxative and L-glutamine groups. Conclusion: L-glutamine, administered in enteral solutions in rats with induced acute pancreatitis, may reduce septic complications by decreasing the bacterial translocation rate. Similar effects are also produced by laxatives through reduction in the luminal bacterial population.
  • Küçük Resim Yok
    Öğe
    Effects of the Trans-9 18:1 Octadecenoic Acid Isomer on Rat Liver Membrane Na+/K+ ATPase Enzyme Activity Plasma Lipoproteins
    (2010) Kaya, Ayşe; Topçu, Cemile; Gürbilek, Mehmet; Aköz, Mehmet; Ünaldı, M.
    Purpose: The consumption of trans-fatty acids is associated with increasing coronary heart disease risk. When oils are partially hydrogenised to margarine form, trans isomers of fatty acids are formed. In this study we investigated the effects of dietary trans-9 18:1 octadecenoic acid isomer on liver cell membrane Na+/K+ ATPase enzyme activity, fat composition and lipid metabolism. Method and Material: This study was conducted on two groups of rats, study and control. Study group was given 50 mg/day trans-9 18:1 octadecenoic acid isomers for ten days. Fatty acid composition of serum and liver tissue was analyzed using gas chromatography Results: Study group showed trans-9 18:1 fatty acid presence while control group did not, thus trans-9 18:1 octadecenoic acid isomer is not a naturally occurring compound. The meaningful increase of C18:2 6n fatty acid and meaningful decrease of C20:4 6n in study group show that trans-9 18:1 octadecenoic acid isomers affected desaturation activity and prevented C18:2 6n fatty acid from turning into C20:4 6n. Rats fed with trans-9 18:1 octadecenoic acid showed decreased HDL and HDL2 cholesterol levels. No change was observed in HDL3, LDL, total cholesterol, triglyceride and Na+/K+ ATPase activity. Our findings combined with current literature allow us to conclude; sine TFAs change serum HDL and HDL2 cholesterol levels, nutrition's containing TFA may increase the risk of heart disease. 2010 © TurkJBiochem.com.