The use of optical fractionator to estimate the total number of ependymal cells of the spinal cord in leghorn

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dc.contributor.authorBolat, Durmus
dc.contributor.authorTipirdamaz, Sadettin
dc.date.accessioned2020-03-26T18:31:55Z
dc.date.available2020-03-26T18:31:55Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümüen_US
dc.description.abstractThe aim of this study was to estimate the number of ependymal cells in the spinal cord of Leghorn chicks using optical fractionator technique. Forty weeks old six female and six male Leghorn were used. Animals were anesthetized by administration of xylazineketamine combination and euthanasia was carried out. Saline solution was administered to the animals for draining blood completely from the body. Ten percent formalin saline solution was used as a fixative. Decalcification was performed on dissected vertebral columns using Trichloroacetic acid. Spinal cords were removed from vertebral columns and measured. All cords were cut 1 cm in length, 10 and 11 systematic random sampled tissue samples with a sampling ratio of 1/3 were taken from females and males respectively. One transversal section was taken from each of tissue samples at the thickness of 10 mu m. Optical fractionator was performed on sections to estimate the total number of ependymal cells in the cord. It was found that male Leghorns had more ependymal cells than female Leghorns. The maximum number of ependymal cells was found in the caudal part whereas the minimum number of cells was observed in cervical part of the spinal cord in female. Male animals showed a homogeneous distribution of the ependymal cells. It was thought that sex difference must be considered in studies on spinal cord of Leghorn.en_US
dc.identifier.citationBolat, D., Tıpırdamaz, S. (2012). The Use of Optical Fractionator to Estimate the Total Number of Ependymal Cells of the Spinal Cord in Leghorn. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(2), 129-133.
dc.identifier.endpage133en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage129en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28571
dc.identifier.volume18en_US
dc.identifier.wosWOS:000302228400023en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorTipirdamaz, Sadettin
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEpendymal cellen_US
dc.subjectSpinal corden_US
dc.subjectOptical fractionatoren_US
dc.subjectLeghornen_US
dc.subjectStereologyen_US
dc.titleThe use of optical fractionator to estimate the total number of ependymal cells of the spinal cord in leghornen_US
dc.typeArticleen_US

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