Amperometric Glucose Biosensor Based on Gold-Deposited Polyvinylferrocene Film on Pt Electrode

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dc.contributor.authorSulak, Meral Topçu
dc.contributor.authorGökdoğan, Özlem
dc.contributor.authorGülce, Ahmet
dc.contributor.authorGülce, Handan
dc.date.accessioned2020-03-26T17:03:01Z
dc.date.available2020-03-26T17:03:01Z
dc.date.issued2006
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe preparations and performances of the novel amperometric biosensors for glucose based on immobilized glucose oxidase (GOD) on modified Pt electrodes are described. Two types of modified electrodes for the enzyme immobilization were used in this study, polyvinyl ferrocene (PVF) coated Pt electrode and gold deposited PVF coated Pt electrode. A simple method forthe immobilization of GOD enzyme on the modified electrodes was described. The enzyme electrodes developed in this study were called as PVF-GOD enzyme electrode and PVF-Au-GOD enzyme electrode, respectively. The amperometric responses of the enzyme electrodes were measured at constant potential, which was due to the electrooxidation of enzymatically produced H2O2. The electrocatalytic effects of the polymer. PVF. and the cold particles towards the electrooxidation of the enzymatically generated H2O2 offers sensitive and selective monitoring of glucose. The biosensor based on PVF-Au-GOD electrode has 6.6 times larger maximum current, 3.8 times higher sensitivity and 1.6 times larger linear working portion than those of the biosensor based on PVF-GOD electrode. The effects of the applied potential. the thickness of the polymeric film. the amount of the immobilized enzyme, pH. the amount of the deposited An, temperature and substrate concentration on the responses of the biosensors were investigated. The optimum pH was found to be pH 7.4 at 25 degrees C. Finally the effects of interferents, stability of the biosensors and applicability to serum analysis of the biosensor were also investigated.en_US
dc.identifier.citationSulak, M. T., Gökdoğan, Ö., Gülce, A., Gülce, H., (2006). Amperometric Glucose Biosensor Based on Gold Deposited Polyvinylferrocene Film on Pt Electrode. Biosensors & Bioelectronics, (21), 1719-1726. Doi: 10.1016/j.bios.2005.08.008
dc.identifier.doi10.1016/j.bios.2005.08.008en_US
dc.identifier.endpage1726en_US
dc.identifier.issn0956-5663en_US
dc.identifier.issn1873-4235en_US
dc.identifier.pmid16198102en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1719en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.bios.2005.08.008
dc.identifier.urihttps://hdl.handle.net/20.500.12395/20315
dc.identifier.volume21en_US
dc.identifier.wosWOS:000236317400006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorGökdoğan, Özlem
dc.institutionauthorGülce, Ahmet
dc.institutionauthorGülce, Handan
dc.language.isoenen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPolyvinylferroceneen_US
dc.subjectGold depositionen_US
dc.subjectGlucoseen_US
dc.subjectElectrochemical biosensoren_US
dc.titleAmperometric Glucose Biosensor Based on Gold-Deposited Polyvinylferrocene Film on Pt Electrodeen_US
dc.typeArticleen_US

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