Protection by Free Oxygen Radical Scavenging Enzymes Against Salicylate-İnduced Embryonic Malformations in Vitro

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dc.contributor.authorKarabulut, A. K.
dc.contributor.authorÜlger, H.
dc.contributor.authorPratten, M. K.
dc.date.accessioned2020-03-26T16:36:18Z
dc.date.available2020-03-26T16:36:18Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSalicylates are among the oldest and most widely used drugs and are known to lead to foetal death, growth retardation and congenital abnormalities in experimental animals. In this study, the effects of acetyl salicylic acid (ASA), salicylic acid (SAL) and sodium salicylate (NaSAL) on early organogenesis and the interaction of these molecules with free radicals has been investigated. Postimplantation rat embryos were cultured in vitro from day 9.5 of gestation for 48 hr. ASA, SAL and NaSAL were added to whole rat serum at concentrations between 0.1 and 0.6 mg/ml. Also, the lowest effective concentration of ASA for all parameters (0.3 mg/ml) and the same concentration of NaSAL and SAL was added to the culture media in the presence of superoxide dismutase (SOD) (30 U/ml) or glutathione (0.5 mu mol/ml). The growth and development of embryos was compared and each embryo was evaluated for the presence of any malformations. When compared to growth of control embryos, the salicylates decreased all growth and developmental parameters in a concentration-responsive manner. There was also a concentration-related increase in overall dysmorphology, including the incidence of haematoma in the yolk sac and neural system, open neural tube, abnormal tail torsion and the absence of fore limb bud. When SOD was added in the presence of ASA, growth and developmental parameters were improved and there was a significant decrease in the incidence of malformations. Addition of SOD also decreased the incidence of malformations in the presence of SAL, but did not effect the growth and developmental parameters of SAL and NaSAL. There was no significant difference between the embryos grown in the presence of these three molecules on the addition of glutathione. The effects of salicylates might involve free oxygen radicals by the non-enzymatic production of the highly teratogenic metabolites 2,3- and 2,5-dihydroxybenzoic acid. An enhanced production of these metabolites in embryonic tissues may be directly related to the increased risk of congenital malformations.en_US
dc.identifier.citationKarabulut, A. K., Ülger, H., Pratten, M. K., (2000). Protection by Free Oxygen Radical Scavenging Enzymes Against Salicylate-İnduced Embryonic Malformations in Vitro. Toxicology in Vitro, 14(4), 297-307. Doi: 10.1016/S0887-2333(00)00023-0
dc.identifier.doi10.1016/S0887-2333(00)00023-0en_US
dc.identifier.endpage307en_US
dc.identifier.issn0887-2333en_US
dc.identifier.issue4en_US
dc.identifier.pmid10906436en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage297en_US
dc.identifier.urihttps://dx.doi.org/10.1016/S0887-2333(00)00023-0
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17241
dc.identifier.volume14en_US
dc.identifier.wosWOS:000088330800002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarabulut, A. K.
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxicology in Vitroen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectacetyl salicylic aciden_US
dc.subjectsodium salicylateen_US
dc.subjectembryoen_US
dc.subjectteratogenen_US
dc.subjectfree radicalsen_US
dc.titleProtection by Free Oxygen Radical Scavenging Enzymes Against Salicylate-İnduced Embryonic Malformations in Vitroen_US
dc.typeArticleen_US

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