Sperm cellular and nuclear dynamics associated with bull fertility

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dc.contributor.authorKutchy, Naseer A.
dc.contributor.authorMenezes, Erica S.B.
dc.contributor.authorUğur, Muhammet R.
dc.contributor.authorUl Husna, Asma
dc.contributor.authorElDebaky, Hazem
dc.contributor.authorEvans, Holly C.
dc.contributor.authorBeaty, Emilly
dc.contributor.authorSantos, Fagner C.
dc.contributor.authorTan, Wei
dc.contributor.authorWills, Robert W.
dc.contributor.authorTopper, Einko
dc.contributor.authorKaya, Abdullah
dc.contributor.authorMoura, Arlindo A.
dc.contributor.authorMemili, Erdoğan
dc.date.accessioned2020-03-26T20:20:01Z
dc.date.available2020-03-26T20:20:01Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractThe objective of this study was to ascertain cellular characteristics and the dynamics of the sperm chromatin proteins protamine 1 (PRM1) and protamine 2 (PRM2) in the sperm of Holstein bulls having a different fertility status. Important sperm variables were analyzed using computer-assisted sperm analysis (CASA). Sperm membrane, acrosome status, DNA integrity were also assessed using propidium iodide (PI), fluorescein isothiocyanate conjugated to Arachis hypogaea (FITC-PNA), and acridine orange (AO) followed by flow cytometry. In addition, abundances of PRM1 and PRM2 were analyzed using flow cytometry experiments. Differences in sperm decondensation capacity were assessed in bulls of varying fertility using a decondensation assay. As determined using CASA, average pathway velocity, amplitude of lateral head displacement and straightness were different (P < 0.05) for sperm from high and low fertility bulls. There, however, were no differences between the high and low fertility bulls for characteristics of sperm plasma membrane, acrosome, and DNA integrity (P > 0.05). Relative abundances of PRM1 and PRM2 in sperm from the high and low fertility bulls were inversely related (P < 0.0001). Percentages of decondensed sperm were different between high and low fertility bulls (P < 0.0001) and total numbers of decondensed sperm were greater in low fertility bulls than high fertility bulls (R2 = 0.72). Results of the present study are significant because molecular and morphological phenotypes of sperm that were detected affect fertility in livestock species.en_US
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico Mississippi Agricultural and Forestry Experiment Station, Mississippi State University Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Alabama Commission on Higher Educationen_US
dc.description.sponsorshipThe research was partially supported in part by Mississippi Agricultural and Forestry Experiment Station, Alta Genetics. Inc. , Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil , Foundation for Research and Scientific Development of the State of Ceara , Brazil (FUNCAP) , and by Selcuk University, Konya, Turkey . NAK was funded by the Netaji Subhas-Indian Council of Agricultural Research for International Fellowships 2015–2017, India (NS-ICAR) . ESB Menezes was supported by a competitive fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) of Brazil . MRU was funded by the Turkish Ministry of National Education . Hazem ElDebaky, was funded by Arab Republic of Egypt . Asma Ul Husna was funded by Higher Education Commission of Pakistan under International Research Support Program. MR Ugur was founded by a competitive fellowship from Ministry of National Education of Turkey .en_US
dc.identifier.citationKutchy, N.A.,Menezes, E.S.B., Uğur, M.R., Ul Husna, A., ElDebaky, H., Evans, H. C., Beaty, E., Santos, F. C., Tan, W., Wills, R. W., Topper, E., Kaya, A., Moura, A.A., Memili, E. (2019). Sperm Cellular and Nuclear Dynamics Associated With Bull Fertility. Animal Reproduction Science, 211, 1-11.
dc.identifier.doi10.1016/j.anireprosci.2019.106203en_US
dc.identifier.issn0378-4320en_US
dc.identifier.pmid31785643en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.anireprosci.2019.106203
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38479
dc.identifier.volume211en_US
dc.identifier.wosWOS:000520009800003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Abdullah
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofAnimal Reproduction Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectChromatin decondensationen_US
dc.subjectMale fertilityen_US
dc.subjectNuclear proteinsen_US
dc.subjectProtaminesen_US
dc.subjectSperm DNA integrityen_US
dc.titleSperm cellular and nuclear dynamics associated with bull fertilityen_US
dc.typeArticleen_US

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