Expression dynamics of Integrin Subunit Beta 5 in bovine gametes and embryos imply functions in male fertility and early embryonic development

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dc.authorid0000-0002-8335-5645
dc.contributor.authorVelho, Ana.
dc.contributor.authorWang, Hongfeng.
dc.contributor.authorKoenig, Leslie.
dc.contributor.authorGrant, Kamilah E.
dc.contributor.authorMenezes, Erika S.
dc.contributor.authorKaya, Abdullah.
dc.contributor.authorMoura, Arlindo.
dc.contributor.authorMemili, Erdogan.
dc.date.accessioned2020-03-26T20:14:14Z
dc.date.available2020-03-26T20:14:14Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractIntegrins have been shown to act as signalling receptors, and they primarily recognise extracellular matrix ligands on the oocyte surface. However, their possible roles in oocyte activation and embryo development are not clearly understood. The objectives of this study were to evaluate expression of Integrin Subunit Beta 5 (ITG beta 5) in bovine sperm, oocytes, and early embryos and to ascertain the evolutionary conservation of ITG beta 5. To accomplish these objectives, we used western blotting to study expression levels of ITG beta 5 protein in sperm and RT-qPCR to determine expression levels of ITG beta 5 transcripts in oocytes and embryos. We have also used bioinformatic analysis to determine the evolutionary conservation of the ITG beta 5 protein among various species. Western blotting showed that ITG beta 5 protein was detectable in bull sperm. Moreover, results of RT-qPCR showed that levels of ITG beta 5 were significantly higher in the two-cell embryos, followed by the 8-16-cell embryos. However, no significant difference in expression levels were noted for the morula and blastocyst stages as compared to MII oocytes. Bioinformatic analysis revealed that ITG beta 5 is conserved among various species. We conclude that expression of ITG beta 5 in bovine gametes and embryos implies an important role in fertilisation and embryogenesis.en_US
dc.description.sponsorshipMississippi Agricultural Forestry Experiment Station; Brazilian Research Council; Selcuk UniversitySelcuk University; Merial Veterinary Scholars Programme; College of Veterinary Medicine of Mississippi State University; Improvement of Higher Educationen_US
dc.description.sponsorshipMississippi Agricultural Forestry Experiment Station; Brazilian Research Council; Selcuk University; Improvement of Higher Education; Merial Veterinary Scholars Programme; College of Veterinary Medicine of Mississippi State Universityen_US
dc.identifier.citationVelho, A., Wang, H., Koenig, L., Grant, K. E., Menezes, E. S., Kaya, A., Moura, A., Memili, E. (2019). Expression Dynamics of Integrin Subunit Beta 5 in Bovine Gametes and Embryos Imply Functions in Male Fertility and Early Embryonic Development. Andrologia, 51(7), e13305.
dc.identifier.doi10.1111/and.13305en_US
dc.identifier.issn0303-4569en_US
dc.identifier.issn1439-0272en_US
dc.identifier.issue7en_US
dc.identifier.pmid31090238en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1111/and.13305
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37844
dc.identifier.volume51en_US
dc.identifier.wosWOS:000474289500004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Abdullah.
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofANDROLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdevelopmental biologyen_US
dc.subjectembryoen_US
dc.subjectfertilizationen_US
dc.subjectgene expressionen_US
dc.titleExpression dynamics of Integrin Subunit Beta 5 in bovine gametes and embryos imply functions in male fertility and early embryonic developmenten_US
dc.typeArticleen_US

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