In Vitro Determination of 6PGD Enzyme Activity Purified from Lake Van Fish (Chalcalburnus tarichii Pallas, 1811) Liver Exposed to Pesticides
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present study, the effect of methidathion, cypermethrin, and deltamethrin pesticides on Lake Van fish (Chalcalburnus tarichii Pallas, 1811) liver 6-phosphogluconate dehydrogenase enzyme activity was investigated due to the fact that these pesticides are extensively used to improve agricultural productivity in the Van region. 2',5'-ADP Sepharose 4B affinity chromatography was used to purify 6-phosphogluconate dehydrogenase enzyme from fish liver and SDS-PAGE technique was used to control the purity of this enzyme. The in vitro effect of methidathion, cypermethrin, and deltamethrin pesticides on the enzyme activity was investigated. The enzyme was purified 1,050-fold with specific activity of 27.04 EU/mg protein. Moreover, K-i constants of methidathion, cypermethrin, and deltamethrin were to be 3.294 +/- A 0.215, 0.718 +/- A 0.095, and 0.084 +/- A 0.009 mM respectively. The IC50 value were estimated as 9.95 x 10(-5) +/- A 0.1844 x 10(-5) mM for methidathion, 1.01 x 10(-4) +/- A 0.01413 x 10(-4) mM for cypermethrin, and 4.43 x 10(-6) +/- A 0.05653 x 10(-6) mM for deltamethrin. In conclusion, deltamethrin inhibits the enzyme activity more than methidathion and cypermethrin.
Açıklama
Anahtar Kelimeler
6-Phosphogluconate dehydrogenase, Purification, Pesticide, Inhibition, Characteristics
Kaynak
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
91
Sayı
5
