In Vitro Determination of 6PGD Enzyme Activity Purified from Lake Van Fish (Chalcalburnus tarichii Pallas, 1811) Liver Exposed to Pesticides

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dc.contributor.authorGuler, Muhammet
dc.contributor.authorKivanc, M. Riza
dc.contributor.authorTurkoglu, Vedat
dc.contributor.authorBasi, Zehra
dc.contributor.authorKivrak, Hilal
dc.date.accessioned2020-03-26T18:42:09Z
dc.date.available2020-03-26T18:42:09Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, the effect of methidathion, cypermethrin, and deltamethrin pesticides on Lake Van fish (Chalcalburnus tarichii Pallas, 1811) liver 6-phosphogluconate dehydrogenase enzyme activity was investigated due to the fact that these pesticides are extensively used to improve agricultural productivity in the Van region. 2',5'-ADP Sepharose 4B affinity chromatography was used to purify 6-phosphogluconate dehydrogenase enzyme from fish liver and SDS-PAGE technique was used to control the purity of this enzyme. The in vitro effect of methidathion, cypermethrin, and deltamethrin pesticides on the enzyme activity was investigated. The enzyme was purified 1,050-fold with specific activity of 27.04 EU/mg protein. Moreover, K-i constants of methidathion, cypermethrin, and deltamethrin were to be 3.294 +/- A 0.215, 0.718 +/- A 0.095, and 0.084 +/- A 0.009 mM respectively. The IC50 value were estimated as 9.95 x 10(-5) +/- A 0.1844 x 10(-5) mM for methidathion, 1.01 x 10(-4) +/- A 0.01413 x 10(-4) mM for cypermethrin, and 4.43 x 10(-6) +/- A 0.05653 x 10(-6) mM for deltamethrin. In conclusion, deltamethrin inhibits the enzyme activity more than methidathion and cypermethrin.en_US
dc.description.sponsorshipadministrative units of the scientific research projects of Van Yuzuncu Yil UniversityYuzuncu Yil Universityen_US
dc.description.sponsorshipAuthors would like to thank the administrative units of the scientific research projects of Van Yuzuncu Yil University for the financial support.en_US
dc.identifier.doi10.1007/s00128-013-1096-2en_US
dc.identifier.endpage564en_US
dc.identifier.issn0007-4861en_US
dc.identifier.issn1432-0800en_US
dc.identifier.issue5en_US
dc.identifier.pmid24057299en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage560en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00128-013-1096-2
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29565
dc.identifier.volume91en_US
dc.identifier.wosWOS:000325701700014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofBULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subject6-Phosphogluconate dehydrogenaseen_US
dc.subjectPurificationen_US
dc.subjectPesticideen_US
dc.subjectInhibitionen_US
dc.subjectCharacteristicsen_US
dc.titleIn Vitro Determination of 6PGD Enzyme Activity Purified from Lake Van Fish (Chalcalburnus tarichii Pallas, 1811) Liver Exposed to Pesticidesen_US
dc.typeArticleen_US

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