Use of the fertilized hen's egg in the evaluation of embryotoxicity of dental alloys
Yükleniyor...
Dosyalar
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY-LISS
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, embryotoxic effects of five commercially available dental alloys were investigated by using fertilized hens' eggs. One sample of each alloy was conditioned in one of the conditioning media, physiological saline (PS), 0.1 M phosphate-buffered saline (PBS, pH 7.4), and 0.1 M protein (3% bovine serum albumin, BSA) containing PBS (pH 6.8), respectively. The test solutions were injected into the fertilized hen's eggs via air sac at the beginning of the incubation. Various concentrations of a highly embryotoxic substance, AFB(1), was also used as positive control test material. Mortality of the AFB(1) treatment groups increased with increasing concentrations of AFB(1), and the mortality values were significantly (p < 0.05) higher than those of the controls and eluent injected groups. The eluents of five commercially available dental alloys tested in the study did not have significant embryotoxic effects. Cu- and Pd-containing alloys displayed relatively high but statistically insignificant embryotoxic effects. Chick embryo might be used in determination of the embryotoxic effects of the dental alloys with its several advantages. Nevertheless, the test should be further standardized and new methods such as micronucleus test showing possible genotoxic effects of the materials should be used. (C) 2004 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
embryotoxicity, CHEST, chick embryo, dental alloy, AFB(1)
Kaynak
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
72B
Sayı
2
