Determination of Intracellular Efficacies of Azithromycin Against Leishmania Major Infection in Human Neutrophils in Vitro
Yükleniyor...
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
JOHN WILEY & SONS LTD
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Azithromycin is one of a new class of antibiotics known as azalides. Azithromycin has high tissue affinity and this feature is thought to be due to the presence of two basic tertiary amine groups. Leishmania major, one of the causative agents of cutaneous leishmaniosis, is an obligate intracellular parasite. In this in vitro study, the potential anti-leishmanial effect of azithromycin upon intracellular forms namely the amastigote of L. major in mice peritoneal macrophages was investigated. L. major promastigotes were propagated in RPMI- 1640 supplemented with 20% fetal calf serum in the log phase. The percentage of phagocytosis and microbiacidal activity of azithromycin on macrophages was assessed in the control and study groups by fluorescence microscopy, using acridine orange. Our results showed that at all the concentrations used (0.05, 0.1, 0.3, 0.6 mugml(-1)) azithromycin had no inhibitory effect on the phagocytic capacity of mouse peritoneal macrophages. Although no significant difference was observed for leishmaniacidal activity between the study and the control groups at a concentration of 0.05 mug ml(-1) (p > 0.05), a significant (p < 0.05) increase in leishmaniacidal activity was detected at 0.1, 0.3 and 0.6 mug ml(-1). As a result, azithromycin does not provide any contribution to the phagocytosis of L. major promastigotes in macrophages in vitro, but it increases the intracellular killing rates of amastigotes. These results suggest that it has a potential anti-leishmanial effect, and may provide a significant advantage in the treatment of the disease. Copyright (C) (C) 2002 John Wiley Sons, Ltd.
Açıklama
Anahtar Kelimeler
Azithromycin, Leishmania major, Neutrophil, İntracellular
Kaynak
Cell Biochemistry and Function
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
21
Sayı
1
Künye
Tanyüksel, M., Baş, A. L., Araz, E., Aybay, C., (2003). Determination of Intracellular Efficacies of Azithromycin Against Leishmania Major Infection in Human Neutrophils in Vitro. Cell Biochemistry and Function, 21(1), 93-96. DOİ: 10.1002/cbf.1004
