Determination of Intracellular Efficacies of Azithromycin Against Leishmania Major Infection in Human Neutrophils in Vitro

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dc.contributor.authorTanyüksel, Mehmet
dc.contributor.authorBaş, A. Levent
dc.contributor.authorAraz, Engin
dc.contributor.authorAybay, Cemalettin
dc.date.accessioned2020-03-26T16:45:33Z
dc.date.available2020-03-26T16:45:33Z
dc.date.issued2003
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAzithromycin is one of a new class of antibiotics known as azalides. Azithromycin has high tissue affinity and this feature is thought to be due to the presence of two basic tertiary amine groups. Leishmania major, one of the causative agents of cutaneous leishmaniosis, is an obligate intracellular parasite. In this in vitro study, the potential anti-leishmanial effect of azithromycin upon intracellular forms namely the amastigote of L. major in mice peritoneal macrophages was investigated. L. major promastigotes were propagated in RPMI- 1640 supplemented with 20% fetal calf serum in the log phase. The percentage of phagocytosis and microbiacidal activity of azithromycin on macrophages was assessed in the control and study groups by fluorescence microscopy, using acridine orange. Our results showed that at all the concentrations used (0.05, 0.1, 0.3, 0.6 mugml(-1)) azithromycin had no inhibitory effect on the phagocytic capacity of mouse peritoneal macrophages. Although no significant difference was observed for leishmaniacidal activity between the study and the control groups at a concentration of 0.05 mug ml(-1) (p > 0.05), a significant (p < 0.05) increase in leishmaniacidal activity was detected at 0.1, 0.3 and 0.6 mug ml(-1). As a result, azithromycin does not provide any contribution to the phagocytosis of L. major promastigotes in macrophages in vitro, but it increases the intracellular killing rates of amastigotes. These results suggest that it has a potential anti-leishmanial effect, and may provide a significant advantage in the treatment of the disease. Copyright (C) (C) 2002 John Wiley Sons, Ltd.en_US
dc.identifier.citationTanyüksel, M., Baş, A. L., Araz, E., Aybay, C., (2003). Determination of Intracellular Efficacies of Azithromycin Against Leishmania Major Infection in Human Neutrophils in Vitro. Cell Biochemistry and Function, 21(1), 93-96. DOİ: 10.1002/cbf.1004
dc.identifier.doi10.1002/cbf.1004en_US
dc.identifier.endpage96en_US
dc.identifier.issn0263-6484en_US
dc.identifier.issue1en_US
dc.identifier.pmid12579528en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://dx.doi.org/10.1002/cbf.1004
dc.identifier.urihttps://hdl.handle.net/20.500.12395/18411
dc.identifier.volume21en_US
dc.identifier.wosWOS:000181097100014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBaş, A. Levent
dc.language.isoenen_US
dc.publisherJOHN WILEY & SONS LTDen_US
dc.relation.ispartofCell Biochemistry and Functionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAzithromycinen_US
dc.subjectLeishmania majoren_US
dc.subjectNeutrophilen_US
dc.subjectİntracellularen_US
dc.titleDetermination of Intracellular Efficacies of Azithromycin Against Leishmania Major Infection in Human Neutrophils in Vitroen_US
dc.typeArticleen_US

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