Enhancement of the Activity and Enantioselectivity of Lipase by Sol-Gel Encapsulation Immobilization onto beta-cyclodextrin-Based Polymer
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Candida rugosa lipase was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of beta-cyclodextrin-based polymer. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yield of the encapsulated lipase was 65 U/g, which is 7.5 times higher than that of the covalently immobilized lipase. The beta-cyclodextrin-based encapsulated lipases had higher conversion and enantioselectivity compared with covalently immobilized lipase. The study confirms an excellent enantioselectivity (E > 300) for the encapsulated lipase with an enantiomeric excess value of 98% for S-naproxen.
Açıklama
Anahtar Kelimeler
Candida rugosa lipase, beta-cyclodextrin, Sol-gel encapsulation, Enantioselective hydrolysis, S-naproxen
Kaynak
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
166
Sayı
8
