Enhancement of the Activity and Enantioselectivity of Lipase by Sol-Gel Encapsulation Immobilization onto beta-cyclodextrin-Based Polymer

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dc.contributor.authorYilmaz, Elif
dc.contributor.authorSezgin, Mehmet
dc.date.accessioned2020-03-26T18:25:29Z
dc.date.available2020-03-26T18:25:29Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCandida rugosa lipase was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of beta-cyclodextrin-based polymer. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yield of the encapsulated lipase was 65 U/g, which is 7.5 times higher than that of the covalently immobilized lipase. The beta-cyclodextrin-based encapsulated lipases had higher conversion and enantioselectivity compared with covalently immobilized lipase. The study confirms an excellent enantioselectivity (E > 300) for the encapsulated lipase with an enantiomeric excess value of 98% for S-naproxen.en_US
dc.description.sponsorshipScientific Research Projects Foundation of Selcuk University (SUBAP)Selcuk University [08101024]en_US
dc.description.sponsorshipThe authors thank the Scientific Research Projects Foundation of Selcuk University (SUBAP grant number 08101024) for financial support of this work produced from a part of E. Yilmaz's Ph.D. thesis.en_US
dc.identifier.doi10.1007/s12010-012-9621-zen_US
dc.identifier.endpage1940en_US
dc.identifier.issn0273-2289en_US
dc.identifier.issn1559-0291en_US
dc.identifier.issue8en_US
dc.identifier.pmid22383051en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1927en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s12010-012-9621-z
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28013
dc.identifier.volume166en_US
dc.identifier.wosWOS:000302771000004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectbeta-cyclodextrinen_US
dc.subjectSol-gel encapsulationen_US
dc.subjectEnantioselective hydrolysisen_US
dc.subjectS-naproxenen_US
dc.titleEnhancement of the Activity and Enantioselectivity of Lipase by Sol-Gel Encapsulation Immobilization onto beta-cyclodextrin-Based Polymeren_US
dc.typeArticleen_US

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