Immobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatum
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Sporopollenin is a natural polymer obtained from Lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. In this study, immobilization of lipase from Candida rugosa (CRL) on sporopollenin by adsorption method is reported for the first time. Besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. It has been observed that under the optimum conditions (Spo-E-(0.3)), the specific activity of the immobilized lipase on the sporopollenin by adsorption was 16.3 U/mg protein, which is 0.46 times less than that of the free lipase (35.6 U/mg protein). The pH and temperature of immobilized enzyme were optimized, which were 6.0 and 40 degrees C respectively. Kinetic parameters V-max and K-m were also determined for the immobilized lipase. It was observed that there is an increase of the K-m value (7.54 mM) and a decrease of the V-max value (145.0 U/mg-protein) comparing with that of the free lipase. (C) 2009 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Sporopollenin, Physical adsorption, Lycopodium clavatum, Immobilization, Lipase
Kaynak
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
45
Sayı
3
