Immobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatum

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dc.contributor.authorTutar, Havva
dc.contributor.authorYılmaz, Elif
dc.contributor.authorPehlivan, Erol
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T17:38:54Z
dc.date.available2020-03-26T17:38:54Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSporopollenin is a natural polymer obtained from Lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. In this study, immobilization of lipase from Candida rugosa (CRL) on sporopollenin by adsorption method is reported for the first time. Besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. It has been observed that under the optimum conditions (Spo-E-(0.3)), the specific activity of the immobilized lipase on the sporopollenin by adsorption was 16.3 U/mg protein, which is 0.46 times less than that of the free lipase (35.6 U/mg protein). The pH and temperature of immobilized enzyme were optimized, which were 6.0 and 40 degrees C respectively. Kinetic parameters V-max and K-m were also determined for the immobilized lipase. It was observed that there is an increase of the K-m value (7.54 mM) and a decrease of the V-max value (145.0 U/mg-protein) comparing with that of the free lipase. (C) 2009 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University (BAP), Konya, TurkeySelcuk Universityen_US
dc.description.sponsorshipWe thank the Scientific Research Foundation of Selcuk University (BAP), Konya, Turkey for financial support of this work.en_US
dc.identifier.doi10.1016/j.ijbiomac.2009.06.014en_US
dc.identifier.endpage320en_US
dc.identifier.issn0141-8130en_US
dc.identifier.issn1879-0003en_US
dc.identifier.issue3en_US
dc.identifier.pmid19583977en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage315en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2009.06.014
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23599
dc.identifier.volume45en_US
dc.identifier.wosWOS:000269393500019en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSporopolleninen_US
dc.subjectPhysical adsorptionen_US
dc.subjectLycopodium clavatumen_US
dc.subjectImmobilizationen_US
dc.subjectLipaseen_US
dc.titleImmobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatumen_US
dc.typeArticleen_US

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