Selective gray and white matter staining of the horse spinal cord
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
KAFKAS UNIV, VETERINER FAKULTESI DERGISI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 pm sections to be stained separately with the different staining methods. Six different staining methods, including Modified May - Grunwald - Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson's trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.
Açıklama
Anahtar Kelimeler
Gray matter ratio, Horse, Imaging, Spinal cord, Stain comparison
Kaynak
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
18
Sayı
2
Künye
Bolat, D., Bahar, S., Sur, E., Selcuk, M. L., Tıpırdamaz, S. (2012). Selective Gray and White Matter Staining of the Horse Spinal Cord. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(2), 249-254.
