Selective gray and white matter staining of the horse spinal cord

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dc.contributor.authorBolat, Durmus
dc.contributor.authorBahar, Sadullah
dc.contributor.authorSur, Emrah
dc.contributor.authorSelcuk, Muhammet L.
dc.contributor.authorTipirdamaz, Sadettin
dc.date.accessioned2020-03-26T18:31:16Z
dc.date.available2020-03-26T18:31:16Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümüen_US
dc.description.abstractThe ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 pm sections to be stained separately with the different staining methods. Six different staining methods, including Modified May - Grunwald - Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson's trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.en_US
dc.identifier.citationBolat, D., Bahar, S., Sur, E., Selcuk, M. L., Tıpırdamaz, S. (2012). Selective Gray and White Matter Staining of the Horse Spinal Cord. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(2), 249-254.
dc.identifier.endpage254en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue2en_US
dc.identifier.startpage249en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28391
dc.identifier.volume18en_US
dc.identifier.wosWOS:000302198700013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorBahar, Sadullah
dc.institutionauthorSur, Emrah
dc.institutionauthorSelcuk, Muhammet L.
dc.institutionauthorTipirdamaz, Sadettin
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGray matter ratioen_US
dc.subjectHorseen_US
dc.subjectImagingen_US
dc.subjectSpinal corden_US
dc.subjectStain comparisonen_US
dc.titleSelective gray and white matter staining of the horse spinal corden_US
dc.typeArticleen_US

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