Determination of Intracellular (Neutrophil and Monocyte) Concentrations of Free and Liposome Encapsulated Ampicillin in Sheep

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Küçük Resim

Tarih

2006

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Czech Academy Agricultural Sciences

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

In the current study, intracellular ( neutrophil and monocyte) concentrations of free and liposome encapsulated ampicillin in sheep were investigated. Free ampicillin ( 5 mg/kg b.w.) and liposome encapsulated ampicillin ( 5 mg/kg b.w.) were administered as a bolus intravenous injection to sheep. After the injections, blood samples ( 5 ml) were collected into tubes from v. jugularis at 10, 30, 60 minutes and 2, 4 and 8 hours. Neutrophils and monocytes were isolated, and lysed in distilled water. Ampicillin concentrations were measured by high performance liquid chromatography. The results indicate that liposome encapsulated ampicillin caused the higher intracellular concentrations within neutrophil ( ratio of liposome encapsulated ampicillin/free ampicillin; from 1.393 to 5.416) and monocyte ( ratio of liposome encapsulated ampicillin/free ampicillin; from 0.973 to 2.906) cells than free ampicillin, and liposome encapsulated ampicillin existed a longer length of time within neutrophil ( 4 hours) and monocyte ( 4 hours) cells than free ampicillin ( 60 minutes), as well. This formulation may be beneficial, in that the treatment of intracellular infections are caused by sensitive bacteria.

Açıklama

Anahtar Kelimeler

ampicillin, liposome, neutrophil, monocyte, intracellular concentrations

Kaynak

Veterinarni Medicina

WoS Q Değeri

Q3

Scopus Q Değeri

Q3

Cilt

51

Sayı

2

Künye

Yazar, E., Baş, A. L., Birdane, Y. O., Yapar, K., Elmas, M., Traş, B., (2006). Determination of Intracellular (Neutrophil and Monocyte) Concentrations of Free and Liposome Encapsulated Ampicillin in Sheep. Veterinarni Medicina, 51(2), 51-54. Doi: 10.17221/5517-VETMED