Cholesterol-Loaded Cyclodextrin Enhances Osmotic Tolerance and Inhibits the Acrosome Reaction in Rabbit Spermatozoa

Basit Öğe Kaydı
dc.contributor.authorAksoy, Melih
dc.contributor.authorAkman, Orhan
dc.contributor.authorLehimcioğlu, Necdet Cankat
dc.contributor.authorErdem, Hüseyin
dc.date.accessioned2020-03-26T17:47:25Z
dc.date.available2020-03-26T17:47:25Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe effects of cholesterol-loaded cyclodextrin (CLC) treatment on the osmotic tolerance and ability to undergo the acrosome reaction of rabbit spermatozoa, with an unusually high cholesterol/phospholipid ratio in plasma membranes, were examined in two successive experiments. In the first experiment, CLC-pretreated and untreated sperm cells were exposed for 15 min to one of five fructose solutions, adjusted to 20, 80, 290, 500 or 1500 mOsm/L. After the anisoosmotic challenge, the integrity of sperm membranes in the CLC-supplemented (at a dose level of 3 mg/120 x 10(6) spermatozoa) and control groups was estimated by a modified hypoosmotic swelling test (HOST) associated with a supravital eosin staining test (HE-test). In the second part of the study, the influence of cholesterol supplementation on the acrosome reaction of sperm cells stimulated by either calcium ionophore A23187 (CI) or lysophosphatidylcholine (LPC) was evaluated. CLC pretreatment increased viable and live-HOST-responsive sperm rates (P<0.01) after incubation in anisoosmotic solutions varying from 80 to 1500 mOsm/L However, CLC supplementation did not influence the percentage of HOST-responsive sperm cells (P>0.05). A significant interaction was determined between CLC pretreatment and the level of osmotic pressure in maintaining the functional and physical integrities of sperm membranes undergoing osmotic challenges. Both CI and LPC successfully induced the acrosome reaction in rabbit spermatozoa (P<0.001). Compared with CI, LPC was more effective (P<0.0001). CLC pretreatment resulted in a significant reduction (P<0.01) in the percentage of acrosome reacted sperm cells irrespective of the inducing agent, either CI or LPC. In conclusion, CLC treatment enhanced the anisoosmotic tolerance of rabbit spermatozoa and reduced their ability to undergo the acrosome reaction after stimulation by CI or LPC.en_US
dc.identifier.citationAksoy, M., Akman, O., Lehimcioğlu, N. C., Erdem, H., (2010). Cholesterol-Loaded Cyclodextrin Enhances Osmotic Tolerance and Inhibits the Acrosome Reaction in Rabbit Spermatozoa. Animal Reproduction Science, (120), 166-172. Doi: 10.1016/j.anireprosci.2010.02.014
dc.identifier.doi10.1016/j.anireprosci.2010.02.014en_US
dc.identifier.endpage172en_US
dc.identifier.issn0378-4320en_US
dc.identifier.pmid20304567en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage166en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.anireprosci.2010.02.014
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24695
dc.identifier.volume120en_US
dc.identifier.wosWOS:000278672600023en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErdem, Hüseyin
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCholesterolen_US
dc.subjectCyclodextrinen_US
dc.subjectSpermatozoaen_US
dc.subjectRabbiten_US
dc.subjectOsmotic toleranceen_US
dc.subjectAcrosome reactionen_US
dc.titleCholesterol-Loaded Cyclodextrin Enhances Osmotic Tolerance and Inhibits the Acrosome Reaction in Rabbit Spermatozoaen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
4695.pdf
Boyut:
343.99 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Klinik Bilimler/Makale Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu