Increased glucose uptake and insulin binding activity of nerium oleander in hepatocytes and adipocytes

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dc.contributor.authorYazihan, Nuray
dc.contributor.authorBas, Ahmet Levent
dc.contributor.authorErmis, Ezgi
dc.contributor.authorDemirci, Sule
dc.contributor.authorUney, Kamil
dc.date.accessioned2020-03-26T18:42:10Z
dc.date.available2020-03-26T18:42:10Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümüen_US
dc.description.abstractType 2 diabetes mellitus (DM) affects a large population worldwide. DM is often considered as a syndrome of disordered glucose metabolism. Current conventional drug therapies for DM are usually insufficient and medicinal herbs with antihyperglycemic activities are increasingly sought by diabetic patients and health care professionals. Nerium Oleander (N.O.) is known to be effective in lowering of postprandial blood glucose in DM patients as a folk remedy. In this study we aimed to evaluate effect of N.O. distillate in glucose uptake activity of hepatocytes and adipocytes. The human hepatoma cells Hep3B and mouse adipocyte 3T3-L1 cells were cultured. Depending on the groups, different concentrations insulin (1, 10, 20 IU/ml) and N.O. (0.1, 1, 10, 50 mu g/ml) were added to medium for 48 h. Cellular toxicity and proliferation were evaluated by LDH secretion levels and MU test. A metabolizable fluorescent derivative of glucose, 2-NBDG and FITC-insulin were used for glucose uptake and insulin binding activity. Insulin increased cellular proliferation and decreased LDH leakage and apoptosis in both cell types. Lower dosages of N.O. has no significant effect on apoptosis and cell number while at the highest dosages minimal cytotoxicity was seen mainly in adipocytes. Main effect of N.O. treatment was increased glucose uptake in Hep3B and 3T3-L1 cells (P<0.001). Our results showed that N.O. may be offered as new approaches to treatment of type 2 diabetes by modulating cellular glucose uptake.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [107 O 222]en_US
dc.description.sponsorshipThis research was granted by The Scientific and Technological Research Council of Turkey (TUBITAK, Project No:107 O 222)en_US
dc.identifier.citationYazıhan, N., Bas, A. L., Ermis, E., Demirci, S., Uney, K. (2013). Increased Glucose Uptake and Insulin Binding Activity of Nerium Oleander in Hepatocytes and Adipocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(1), 25-30.
dc.identifier.doi10.9775/kvfd.2012.7044en_US
dc.identifier.endpage30en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://dx.doi.org/10.9775/kvfd.2012.7044
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29570
dc.identifier.volume19en_US
dc.identifier.wosWOS:000314082300005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorBas, Ahmet Levent
dc.institutionauthorUney, Kamil
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectNerium oleanderen_US
dc.subjectHepatocytesen_US
dc.subjectAdipocytesen_US
dc.subjectGlucose uptakeen_US
dc.subjectInsulin bindingen_US
dc.subjectType II diabetes mellitusen_US
dc.titleIncreased glucose uptake and insulin binding activity of nerium oleander in hepatocytes and adipocytesen_US
dc.typeArticleen_US

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