Encapsulation of lipase using magnetic fluorescent calix[4]arene derivatives; improvement of enzyme activity and stability
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE BV
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, iron magnetic nanoparticles capped with fluorescent calixarene derivatives (Fe3O4@Calix-2 and Fe3O4@Calix-3) were prepared in one-step using coprecipitation of Fe(II) and Fe(III) in basic solution. Different techniques were used to characterize the synthesized magnetic nanoparticles, such as Fourier Transform Infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and confocal microscopy. Candida rugosa was encapsulated on synthesized nanoparticles following sol-gel method. It has been observed that under the optimum conditions, the activity of encapsulated lipase (Fe3O4@Calix-2E) was 119 U/g of support which is 4.1 times more that of the encapsulated lipase without calix[4]arene derivative (Fe3O4@E). Comparative study show that the encapsulated lipase on nanoparticles has higher thermal and operational stability than encapsulated lipase without calix[4]arene derivative. Among these encapsulated lipase nanoparticles, Fe3O4@Calix-2 was capable of effectively catalyze hydrolysis of racemic Flurbiprofen methyl ester with high conversion of 49% and substrate enantiomeric excess (ees) of 85% at optimum pH and temperature. The efficiency of these nanoparticles was assessed by their reusability, for that after five consecutive operational uses these encapsulated lipase nanoparticles retained their conversion ratios up to 38% and 30% respectively, in the hydrolysis of (R,S)-Flurbiprofen methyl ester. The results showed that encapsulated lipases nanoparticle with calixarene moieties lead to increased activity, stability, reusability and enhanced stereoselectivity in kinetic resolution. (C) 2019 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Calixarene, Fluorescent, Fe3O4 nanoparticles, Lipase encapsulation, Flurbiprofen
Kaynak
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
WoS Q DeÄŸeri
Q1
Scopus Q DeÄŸeri
Q1
Cilt
133
Sayı
Künye
Ozyilmaz, E., Cetinguney, S., Yilmaz, M. (2019). Encapsulation of Lipase Using Magnetic Fluorescent Calix [4] Arene Derivatives; Improvement of Enzyme Activity and Stability. International Journal of Biological Macromolecules, 133, 1042-1050.
