Encapsulation of lipase using magnetic fluorescent calix[4]arene derivatives; improvement of enzyme activity and stability

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dc.contributor.authorOzyilmaz, Elif.
dc.contributor.authorCetinguney, Sevilay.
dc.contributor.authorYilmaz, Mustafa.
dc.date.accessioned2020-03-26T20:13:54Z
dc.date.available2020-03-26T20:13:54Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyokimya Bölümüen_US
dc.description.abstractIn this study, iron magnetic nanoparticles capped with fluorescent calixarene derivatives (Fe3O4@Calix-2 and Fe3O4@Calix-3) were prepared in one-step using coprecipitation of Fe(II) and Fe(III) in basic solution. Different techniques were used to characterize the synthesized magnetic nanoparticles, such as Fourier Transform Infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and confocal microscopy. Candida rugosa was encapsulated on synthesized nanoparticles following sol-gel method. It has been observed that under the optimum conditions, the activity of encapsulated lipase (Fe3O4@Calix-2E) was 119 U/g of support which is 4.1 times more that of the encapsulated lipase without calix[4]arene derivative (Fe3O4@E). Comparative study show that the encapsulated lipase on nanoparticles has higher thermal and operational stability than encapsulated lipase without calix[4]arene derivative. Among these encapsulated lipase nanoparticles, Fe3O4@Calix-2 was capable of effectively catalyze hydrolysis of racemic Flurbiprofen methyl ester with high conversion of 49% and substrate enantiomeric excess (ees) of 85% at optimum pH and temperature. The efficiency of these nanoparticles was assessed by their reusability, for that after five consecutive operational uses these encapsulated lipase nanoparticles retained their conversion ratios up to 38% and 30% respectively, in the hydrolysis of (R,S)-Flurbiprofen methyl ester. The results showed that encapsulated lipases nanoparticle with calixarene moieties lead to increased activity, stability, reusability and enhanced stereoselectivity in kinetic resolution. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University, Konya, TurkeySelcuk University [15201094]en_US
dc.description.sponsorshipWe would like to thank the Scientific Research Foundation of Selcuk University, Konya, Turkey (BAP Project No. 15201094) for financial support of this work.en_US
dc.identifier.citationOzyilmaz, E., Cetinguney, S., Yilmaz, M. (2019). Encapsulation of Lipase Using Magnetic Fluorescent Calix [4] Arene Derivatives; Improvement of Enzyme Activity and Stability. International Journal of Biological Macromolecules, 133, 1042-1050.
dc.identifier.doi10.1016/j.ijbiomac.2019.04.182en_US
dc.identifier.endpage1050en_US
dc.identifier.issn0141-8130en_US
dc.identifier.issn1879-0003en_US
dc.identifier.pmid31042560en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1042en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2019.04.182
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37785
dc.identifier.volume133en_US
dc.identifier.wosWOS:000472685700112en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOzyilmaz, Elif.
dc.institutionauthorCetinguney, Sevilay.
dc.institutionauthorYilmaz, Mustafa.
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCalixareneen_US
dc.subjectFluorescenten_US
dc.subjectFe3O4 nanoparticlesen_US
dc.subjectLipase encapsulationen_US
dc.subjectFlurbiprofenen_US
dc.titleEncapsulation of lipase using magnetic fluorescent calix[4]arene derivatives; improvement of enzyme activity and stabilityen_US
dc.typeArticleen_US

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