Development of an Enzyme Immunoassay for the Determination of Ovine Growth Hormone in Plasma

Basit Öğe Kaydı
dc.contributor.authorSerpek, Behiç
dc.contributor.authorHaliloğlu, Seyfullah
dc.date.accessioned2020-03-26T16:36:13Z
dc.date.available2020-03-26T16:36:13Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAbstract: A competitive enzyme immunoassay for the determination of ovine growth hormone (oGH) was developed. The assay is based on (anti-rabbit IgG) goat IgG-coated microtitreplates, anti-oGH from rabbits, biotinyl-oGH and streptavidin-horseradish peroxidase. The assay is done directly with 40 µl of plasma and the calibration graph (90% relative binding at 0.38±0.02 ng ml and 50% relative binding at 3.80±0.18 ng ml) is prepared in plasma without measurable endogenous oGH. Recovery in different plasma samples with added oGH amounted to 96.78 100.03%, and all variabilities were < 10.44. The assay shows no significant binding of oFSH, OLH, OTSH and oPRL. The assay detects high and low plasma oGH levels within the physiological variation as well as changes in plasma oGH after stimulation with growth hormone releasing factor. Hence, it offers a reliable alternative radioimmunoassay. However, a reference method providing evidence of the identity of exogenous oGH will be required for proving illegal treatment with oGH and for forensic purposes.en_US
dc.identifier.citationSerpek, B., Haliloğlu, S., (2000). Development of an Enzyme Immunoassay for the Determination of Ovine Growth Hormone in Plasma. Turkish Journal of Veterinary & Animal Sciences, 24(2), 163-168.
dc.identifier.endpage168en_US
dc.identifier.issn1300-0128en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage163en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17194
dc.identifier.volume24en_US
dc.identifier.wosWOS:000087199100011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorSerpek, Behiç
dc.institutionauthorHaliloğlu, Seyfullah
dc.language.isoenen_US
dc.publisherScientific Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectenzymatic methodsen_US
dc.subjectplasmaen_US
dc.subjectovine growth hormoneen_US
dc.titleDevelopment of an Enzyme Immunoassay for the Determination of Ovine Growth Hormone in Plasmaen_US
dc.title.alternativeKoyun Kan Plazması Büyüme Hormonu Düzeylerinin Enzim İmmuno Assay Yöntemiyle Belirlenmesien_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
7194.pdf
Boyut:
169.91 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu
Vet. Fak. Temel Bilimler/Makale Koleksiyonu