Sperm miR-15a and miR-29b are associated with bull fertility

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dc.authorid0000-0002-8335-5645
dc.contributor.authorMenezes, Erika S. B.
dc.contributor.authorBadial, Peres Ramos.
dc.contributor.authorEl Debaky, Hazem.
dc.contributor.authorUl Husna, Asma.
dc.contributor.authorUgur, Muhammet Rasit.
dc.contributor.authorKaya, Abdullah.
dc.contributor.authorTopper, Einko.
dc.contributor.authorBulla, Camilo.
dc.contributor.authorGrant, Kamilah E.
dc.contributor.authorBolden-Tiller, Olga.
dc.contributor.authorMoura, Arlindo A.
dc.contributor.authorMemili, Erdoğan.
dc.date.accessioned2020-03-26T20:20:28Z
dc.date.available2020-03-26T20:20:28Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractMicroRNAs modulate male fertility by regulating gene expression. In this study, dynamics of sperm miR-15a, miR-29b and miR-34a from high fertility (HF) and low fertility (LF) bulls using RT-qPCR were evaluated. Bioinformatic tools were employed to ascertain genes of interest of the sperm miRNAs. The expression levels of p53, BCL2, BAX and DNMT1 in bull spermatozoa were determined by immunoblotting. MicroRNA levels of miR-15a and miR-29 were higher in LF sires when compared with those present in HF bulls. Expression levels of miR-34a did not differ between the two groups. We found an inverse correlation between miR-15a and bull fertility. MiR29-b was also negatively associated with fertility scores. BCL2 and DNMT1 were higher in HF bulls while BAX was higher in the LF group. Our data showed a positive correlation between BCL2 and bull fertility. In addition, DNMT1 was positively associated with bull fertility. Furthermore, levels of BAX were negatively linked with bull fertility scores. Identification of miRNAs found in the spermatozoa of sires with different in vivo fertility helps understand the alterations in the fertilising capacity from cattle and other mammals. These potential biomarkers can be used in reproductive biotechnology as fertility markers to assess semen quality and predict male fertility.en_US
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of BrazilNational Council for Scientific and Technological Development (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of BrazilCAPES; Higher Education Commission of Pakistan under International Research Support ProgramHigher Education Commission of Pakistan; Arab Republic of Egypt; The Republic of Turkey, Ministry of National Education, Directorate General for Higher and Foreign Education; Alta Genetics, Inc; Mississippi Agricultural Experiment Stationen_US
dc.identifier.citationMenezes, E. S. B., Badial, P. R., Debaky, H. E., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., Memili, E. (2020). Sperm miR-15a and miR-29b are Associated with Bull Fertility. Andrologia, 52(1), 1-11.
dc.identifier.doi10.1111/and.13412en_US
dc.identifier.issn0303-4569en_US
dc.identifier.issn1439-0272en_US
dc.identifier.issue1en_US
dc.identifier.pmid31671225en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1111/and.13412
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38595
dc.identifier.volume52en_US
dc.identifier.wosWOS:000505610200030en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Abdullah.
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofANDROLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectmale fertilityen_US
dc.subjectmicroRNAen_US
dc.subjectreal-time quantitative RT-PCRen_US
dc.subjectspermen_US
dc.titleSperm miR-15a and miR-29b are associated with bull fertilityen_US
dc.typeArticleen_US

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