Untargeted metabolomic profiling, Multivariate Analysis and Biological Evaluation of the true mangrove (Rhizophora mucronata Lam.)

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dc.contributor.authorSadeer, Nabeelah Bibi
dc.contributor.authorRocchetti, Gabriele
dc.contributor.authorSenizza, Biancamaria
dc.contributor.authorMontesano, Domenico
dc.contributor.authorZengin, Gökhan
dc.contributor.authorUysal, Ahmet
dc.contributor.authorJeewon, Rajesh
dc.contributor.authorLucini, Luigi
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2020-03-26T20:19:42Z
dc.date.available2020-03-26T20:19:42Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractCurrently, there is a renewed interest towards the development of plant-based pharmacophores. In this work, 16 extracts prepared from the leaves, twigs, roots and fruits of a hydro-halophyte, Rhizophora mucronata Lam. (Family: Rhizophoraceae), were studied for possible antioxidant activity and the phenolic profiles established. Thereafter, enzymatic inhibitory activities (alpha-amylase, alpha-glucosidase, tyrosinase, acetyl- (AChE), butyrylcholinesterase (BChE), lipase, and elastase) were assessed. The total phenolic, flavonoid, phenolic acid, tannin, flavanol and triterpenoid content were estimated using standard assays. An untargeted metabolomics-based approach, based on ultra-high-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS) followed by multivariate statistics, was then used to comprehensively profile and describe the phenolics present. UHPLC-QTOF-MS allowed for putatively annotating 104 phenolic acids, 103 flavonols, 94 flavones, 71 anthocyanins, 66 tyrosols, 29 lignans, 15 alkylphenols and 10 stilbenes in the extracts. Nine strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus aureus (MRSA), Salmonella enteritidis, Sarcina lutea, Proteus mirabilis, Bacillus cereus and Candida albicans) were then used to investigate the antimicrobial properties. The methanolic twig extract exhibited significant reducing potential towards Cu (II)/Cu (I) and Fe (III)/Fe (II) (1336.88 +/- 15.70 and 710.18 +/- 21.04 mg TE/g, respectively) and was the most potent DPPH radical scavenger (807.07 +/- 6.83 mg TE/g). Additionally, the methanolic twig extract showed significant inhibition against most targeted enzymes. Anti-microbial results showed that all extracts were active against MRSA. Multivariate analysis demonstrated that the phenolic profile of ethyl acetate extracts and leaves were the two most discriminative parameters in terms of solvents and organs, respectively. The present findings indicated that R. mucronata may be further explored for the management/prevention of oxidative stress, neurodegenerative complications and hyperpigmentation.en_US
dc.identifier.citationSadeer, N.B., Rocchetti, G., Senizza, B., Montesano, D., Zengin, G., Uysal, A., Jeewon, R., Lucini, L., Mahomoodally, M.F. (2019). Untargeted Metabolomic Profiling, Multivariate Analysis and Biological Evaluation of the True Mangrove (Rhizophora mucronata Lam.). Antıoxıdants, 8,(10), 1-20.
dc.identifier.doi10.3390/antiox8100489en_US
dc.identifier.issn2076-3921en_US
dc.identifier.issue10en_US
dc.identifier.pmid31623170en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.3390/antiox8100489
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38371
dc.identifier.volume8en_US
dc.identifier.wosWOS:000500759900072en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZengin, Gökhan
dc.institutionauthorUysal, Ahmet
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofANTIOXIDANTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjecttrue mangroveen_US
dc.subjectantimicrobialen_US
dc.subjectantioxidanten_US
dc.subjectphytochemicalsen_US
dc.subjecthyperpigmentationen_US
dc.subjectoxidative stressen_US
dc.subjectenzyme inhibitorsen_US
dc.titleUntargeted metabolomic profiling, Multivariate Analysis and Biological Evaluation of the true mangrove (Rhizophora mucronata Lam.)en_US
dc.typeArticleen_US

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