Detection of Integrated Murine Leukemia Viruses in a Mouse Model of Acute Myeloid Leukemia by Fluorescence in Situ Hybridization Combined With Tyramide Signal Amplification
Yükleniyor...
Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study rt as undertaken to develop a reliable method to enumerate and map somatically acquired, clonal, murine leukemia virus (MuLV) proviral insertions in acute myeloid leukemia (AML) cells from the BXH-2 mouse strain. This was achieved by using fluorescence in situ hybridization combined with tyramide signal amplification (FISH-TSA) and an 8.8 kilobase pair (kb) full-length ecotropic MuLV or 2.0 kb MuLV envelope (env) gene probe. Two-color FISH was utilized combining chromosome-specific probes for regions near the telomere and/or centromere and the MuLV probes. The technique reliably detected germline and somatically acquired, tumor-specific, MuLV proviruses in BXH-2 AML cell lines. It rr as possible to readily verify homozygous insertions at endogenous ecotropic,MuLV loci, Emv1 (chromosome 5), Emv2 (chromosome 8) and a BXH-2 strain-specific locus (chromosome 11). This strategy also verified the presence of molecularly cloned proviral insertions within the mouse Nf1 gene and another locus on distal chromosome II, as well as on chromosome 7 and chromosome 9 in BXH-2 AML cell line B117. The technique rc as also used to detect several new tumor-specific, proviral insertions in BXH-2 AML cell lines.
Açıklama
Anahtar Kelimeler
Kaynak
Cancer Genetics and Cytogenetics
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
121
Sayı
1
Künye
Acar, H., Largaespada, D. A., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., (2000). Detection of Integrated Murine Leukemia Viruses in a Mouse Model of Acute Myeloid Leukemia by Fluorescence in Situ Hybridization Combined With Tyramide Signal Amplification. Cancer Genetics and Cytogenetics, 121(1), 44-51. Doi: 10.1016/S0165-4608(00)00232-6
