Detection of Integrated Murine Leukemia Viruses in a Mouse Model of Acute Myeloid Leukemia by Fluorescence in Situ Hybridization Combined With Tyramide Signal Amplification

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dc.contributor.authorAcar, Hasan
dc.contributor.authorCopeland, Neal G.
dc.contributor.authorGilbert, Debra J.
dc.contributor.authorJenkins, Nancy A.
dc.contributor.authorLargaespada, David A.
dc.date.accessioned2020-03-26T16:36:13Z
dc.date.available2020-03-26T16:36:13Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study rt as undertaken to develop a reliable method to enumerate and map somatically acquired, clonal, murine leukemia virus (MuLV) proviral insertions in acute myeloid leukemia (AML) cells from the BXH-2 mouse strain. This was achieved by using fluorescence in situ hybridization combined with tyramide signal amplification (FISH-TSA) and an 8.8 kilobase pair (kb) full-length ecotropic MuLV or 2.0 kb MuLV envelope (env) gene probe. Two-color FISH was utilized combining chromosome-specific probes for regions near the telomere and/or centromere and the MuLV probes. The technique reliably detected germline and somatically acquired, tumor-specific, MuLV proviruses in BXH-2 AML cell lines. It rr as possible to readily verify homozygous insertions at endogenous ecotropic,MuLV loci, Emv1 (chromosome 5), Emv2 (chromosome 8) and a BXH-2 strain-specific locus (chromosome 11). This strategy also verified the presence of molecularly cloned proviral insertions within the mouse Nf1 gene and another locus on distal chromosome II, as well as on chromosome 7 and chromosome 9 in BXH-2 AML cell line B117. The technique rc as also used to detect several new tumor-specific, proviral insertions in BXH-2 AML cell lines.en_US
dc.description.sponsorshipNCI NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [UO1-CA84221]en_US
dc.identifier.citationAcar, H., Largaespada, D. A., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., (2000). Detection of Integrated Murine Leukemia Viruses in a Mouse Model of Acute Myeloid Leukemia by Fluorescence in Situ Hybridization Combined With Tyramide Signal Amplification. Cancer Genetics and Cytogenetics, 121(1), 44-51. Doi: 10.1016/S0165-4608(00)00232-6
dc.identifier.doi10.1016/S0165-4608(00)00232-6en_US
dc.identifier.endpage51en_US
dc.identifier.issn0165-4608en_US
dc.identifier.issue1en_US
dc.identifier.pmid10958940en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage44en_US
dc.identifier.urihttps://dx.doi.org/10.1016/S0165-4608(00)00232-6
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17192
dc.identifier.volume121en_US
dc.identifier.wosWOS:000088983400008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAcar, Hasan
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofCancer Genetics and Cytogeneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleDetection of Integrated Murine Leukemia Viruses in a Mouse Model of Acute Myeloid Leukemia by Fluorescence in Situ Hybridization Combined With Tyramide Signal Amplificationen_US
dc.typeArticleen_US

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