Comparison of manual and automated nucleic acid extraction methods for detection of peste des petits ruminants virus rna

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dc.contributor.authorSevik, Murat
dc.contributor.authorAvci, Oguzhan
dc.contributor.authorInce, Omer Baris
dc.date.accessioned2020-03-26T19:01:30Z
dc.date.available2020-03-26T19:01:30Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümüen_US
dc.description.abstractPeste des petits ruminants (PPR) is an economically important contagious disease of small ruminants. PCR-based techniques have been successfully used for rapid diagnosis of PPR. The method used for isolation of RNA from tissue samples is an important concern when using reverse transcription-PCR (RT-PCR) methods for the detection of PPR virus (PPRV). In this study, a commercial kit for manual preparation and an automated processing technique for RNA extraction were compared in terms of performance. Thirty-two small ruminants, each from different flocks, with PPR suspect submitted to laboratory were chosen to compare manual and automated extraction methods for the detection of PPRV. Vero cells were used for PPRV isolation. One-step RT-PCR was used for the detection of PPRV RNA. From the 32 submitted samples, CPE was observed in 11 samples. PPRV nucleic acid was detected in 11 of 32 samples that were manually extracted, while viral RNA was detected in 9 of 32 extracts prepared by the robot. Two samples that were negative with automated extraction were weakly positive in manual extraction. RNA quality and quantity were assessed using a spectrophotometer. According to the results, difference in quantity among two methods was statistically significant (P<0.0001, two-tailed paired t-test), and manual extraction method is suitable for detection of low amounts of PPRV RNA in clinical samples.en_US
dc.identifier.citationŞevik, M., Avcı, O., İnce, Ö. B. (2015). Comparison of Manual and Automated Nucleic Acid Extraction Methods for Detection of Peste Des Petits Ruminants Virus RNA. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 271-276.
dc.identifier.doi10.9775/kvfd.2014.12251en_US
dc.identifier.endpage276en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage271en_US
dc.identifier.urihttps://dx.doi.org/10.9775/kvfd.2014.12251
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31954
dc.identifier.volume21en_US
dc.identifier.wosWOS:000349190400020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorAvci, Oguzhan
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRNA purificationen_US
dc.subjectManualen_US
dc.subjectAutomateden_US
dc.subjectQualityen_US
dc.subjectQuantityen_US
dc.subjectRT-PCRen_US
dc.titleComparison of manual and automated nucleic acid extraction methods for detection of peste des petits ruminants virus rnaen_US
dc.typeArticleen_US

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