Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods

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dc.authorid0000-0003-2989-459X
dc.authorid0000-0002-6296-3107
dc.contributor.authorŞensoy, Erhan.
dc.contributor.authorÖznurlu, Yasemin.
dc.date.accessioned2020-03-26T20:13:21Z
dc.date.available2020-03-26T20:13:21Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümüen_US
dc.description.abstractBackground/Aims: The aim of the present study was to determine the changes on the small intestine in mice during pregnancy using histological, enzyme histochemical, and immunohistochemical methods. Materials and Methods: A total of 24 Swiss albino female mice were divided as non-pregnant/control, first week, second week, and third week of pregnancy (n=6). Tissue samples obtained from the duodenum, jejunum, and ileum were processed by means of routine histological techniques and stained with Crossmon's triple staining. Alkaline phosphatase (ALP) was demonstrated with the simultaneous azo-coupling method. Proliferating cell nuclear antigen (PCNA) was demonstrated with the streptavidin-biotin-peroxidase complex method. The numerical data of the parameters were obtained and analyzed statistically. Results: Villus height, villus width, and the rate of villus height/crypt depth were decreased in the duodenum, jejunum, and ileum in the last week of pregnancy compared with the control group. Changes in the crypt depth of the duodenum, jejunum, and ileum in pregnancy were found. The muscle width increased in pregnancy. It was identified that the ALP reactivity statistically significantly increased in the duodenum, jejunum, and ileum in pregnancy. The percentage of PCNA-positive cells in the duodenum, jejunum, and ileum increased in the first and second weeks of pregnancy, whereas it decreased in the third week of pregnancy compared with non-pregnant control animals. Conclusion: In conclusion, villus parameters, ALP reactivity, and percentage of PCNA-positive cells in the small intestine were affected during pregnancy.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects (BAP) Coordinating OfficeSelcuk University [09202056]en_US
dc.description.sponsorshipProvision of materials of our study has been afforded by Selcuk University Scientific Research Projects (BAP) Coordinating Office (Project No: 09202056).en_US
dc.identifier.citationŞensoy, E., Öznurlu, Y. (2019). Determination of the Changes on the Small Intestine of Pregnant Mice by Histological, Enzyme Histochemical, and Immunohistochemical Methods. The Turkish Journal of Gastroenterology, 30(10), 917.
dc.identifier.doi10.5152/tjg.2019.18681en_US
dc.identifier.endpage924en_US
dc.identifier.issn2148-5607en_US
dc.identifier.issue10en_US
dc.identifier.pmid31625934en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage917en_US
dc.identifier.urihttps://dx.doi.org/10.5152/tjg.2019.18681
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37675
dc.identifier.volume30en_US
dc.identifier.wosWOS:000490571100009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖznurlu, Yasemin.
dc.language.isoenen_US
dc.publisherAVESen_US
dc.relation.ispartofTURKISH JOURNAL OF GASTROENTEROLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectALPen_US
dc.subjectmiceen_US
dc.subjectPCNAen_US
dc.subjectpregnancyen_US
dc.subjectvillusen_US
dc.titleDetermination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methodsen_US
dc.typeArticleen_US

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