Dexamethasone and basic-fibroblast growth factor regulate markers of mineralization in cementoblasts in vitro
Yükleniyor...
Dosyalar
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
AMER ACAD PERIODONTOLOGY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: The aim of this study was to determine the effects of basic-fibroblast growth factor (b-FGF) and/or dexamethasone (Dex) on cementoblasts in vitro. Methods: Murine cementoblasts were treated as follows: 1) 5% FBS (fetal bovine serum) + ascorbic acid (AA, 50 mu g/ml, control); 2) 5% FBS+Dex (10(-7)M)+AA; 3) 5% FBS+b-FGF (50 ng/ml)+AA; or 4) 5% FBS+Dex (10(-7) M)+b-FGF (50 ng/ml)+AA and then evaluated by Northern analysis for changes in specific genes and by von Kossa stain for changes in mineral nodule formation. Results: Mitotic activity: b-FGF stimulated DNA synthesis significantly versus negative control. Gene expression: osteocalcin (OCN): Dex or b-FGF or the combination resulted in a decrease in expression versus control. Bone sialoprotein (BSP): Dex increased expression of BSP mRNA levels, b-FGF decreased transcript for BSP at 6 and 24 hours. Long-term (8 days) Dex, b-FGF, or Dex plus b-FGF caused a decrease in BSP expression versus control; osteopontin (OPN): both Dex and b-FGF increased transcripts for OPN seen by 6 hours, with a greater increase noted with b-FGF versus Dex. No apparent additive effect of Dex with b-FGF was noted; matrix gamma-carboxyglutamic acid protein (MGP): b-FGF induced transcripts for MGP and addition of Dex increased this effect, while Dex alone had no effect on expression. Biomineralization: Dex increased cementoblast-mediated biornineralization, while b-FGF blocked this activity, and addition of Dex to b-FGF did not alter FGF associated inhibition. Conclusion: Dex and FGF alone and in combination alter cementoblast behavior, but additional studies are required to determine whether these factors have beneficial effects at the clinical level.
Açıklama
Anahtar Kelimeler
dental cementum, dexamethasone, growth factors, fibroblast
Kaynak
JOURNAL OF PERIODONTOLOGY
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
76
Sayı
9
