Dexamethasone and basic-fibroblast growth factor regulate markers of mineralization in cementoblasts in vitro

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dc.contributor.authorHakkı, Sema S.
dc.contributor.authorNohutçu, Rahime M.
dc.contributor.authorHakkı, Erdoğan E.
dc.contributor.authorBerry, Janice E.
dc.contributor.authorAkkaya, Mahinur S.
dc.contributor.authorSomerman, Martha J.
dc.date.accessioned2020-03-26T16:56:50Z
dc.date.available2020-03-26T16:56:50Z
dc.date.issued2005
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: The aim of this study was to determine the effects of basic-fibroblast growth factor (b-FGF) and/or dexamethasone (Dex) on cementoblasts in vitro. Methods: Murine cementoblasts were treated as follows: 1) 5% FBS (fetal bovine serum) + ascorbic acid (AA, 50 mu g/ml, control); 2) 5% FBS+Dex (10(-7)M)+AA; 3) 5% FBS+b-FGF (50 ng/ml)+AA; or 4) 5% FBS+Dex (10(-7) M)+b-FGF (50 ng/ml)+AA and then evaluated by Northern analysis for changes in specific genes and by von Kossa stain for changes in mineral nodule formation. Results: Mitotic activity: b-FGF stimulated DNA synthesis significantly versus negative control. Gene expression: osteocalcin (OCN): Dex or b-FGF or the combination resulted in a decrease in expression versus control. Bone sialoprotein (BSP): Dex increased expression of BSP mRNA levels, b-FGF decreased transcript for BSP at 6 and 24 hours. Long-term (8 days) Dex, b-FGF, or Dex plus b-FGF caused a decrease in BSP expression versus control; osteopontin (OPN): both Dex and b-FGF increased transcripts for OPN seen by 6 hours, with a greater increase noted with b-FGF versus Dex. No apparent additive effect of Dex with b-FGF was noted; matrix gamma-carboxyglutamic acid protein (MGP): b-FGF induced transcripts for MGP and addition of Dex increased this effect, while Dex alone had no effect on expression. Biomineralization: Dex increased cementoblast-mediated biornineralization, while b-FGF blocked this activity, and addition of Dex to b-FGF did not alter FGF associated inhibition. Conclusion: Dex and FGF alone and in combination alter cementoblast behavior, but additional studies are required to determine whether these factors have beneficial effects at the clinical level.en_US
dc.description.sponsorshipNIDCR NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [DE09532]en_US
dc.identifier.doi10.1902/jop.2005.76.9.1550en_US
dc.identifier.endpage1558en_US
dc.identifier.issn0022-3492en_US
dc.identifier.issn1943-3670en_US
dc.identifier.issue9en_US
dc.identifier.pmid16171446en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1550en_US
dc.identifier.urihttps://dx.doi.org/10.1902/jop.2005.76.9.1550
dc.identifier.urihttps://hdl.handle.net/20.500.12395/19661
dc.identifier.volume76en_US
dc.identifier.wosWOS:000232266600019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAMER ACAD PERIODONTOLOGYen_US
dc.relation.ispartofJOURNAL OF PERIODONTOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdental cementumen_US
dc.subjectdexamethasoneen_US
dc.subjectgrowth factorsen_US
dc.subjectfibroblasten_US
dc.titleDexamethasone and basic-fibroblast growth factor regulate markers of mineralization in cementoblasts in vitroen_US
dc.typeArticleen_US

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