Stability of Urine Specimens Stored with and Without Preservatives At Room Temperature and On Ice Prior to Urinalysis

dc.contributor.authorErcan, Müjgan
dc.contributor.authorAkbulut, Emiş Deniz
dc.contributor.authorAbuşoğlu, Sedat
dc.contributor.authorYılmaz, Fatma Meriç
dc.contributor.authorOğuz, Esra Fırat
dc.contributor.authorTopcuoğlu, Canan
dc.contributor.authorÖztekin, Volkan
dc.contributor.authorBoğdaycıoğlu, Nihal
dc.date.accessioned2020-03-26T19:07:10Z
dc.date.available2020-03-26T19:07:10Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjectives: Laboratories determine the most appropriate approach for the collection and transport of urine specimens. We investigated the effect of a chlorhexidine-based preservative tube on sample stability, compared the results of refrigerated polystyrene tubes with no additives, and investigated the effect of temperature on the performance of preservative tubes. Design and methods: Fresh urine specimen (n = 48) aliquots in BD Vacutainer (R) Plus Urinalysis Preservative Tubes and polystyrene tubes were analyzed on an Iris Diagnostics iQ200. Samples in polystyrene tubes were refrigerated for 4 and 8 h. Four aliquots in preservative tubes were kept at room temperature for 4, 8, 24, and 72 h, while two aliquots were kept on ice for 4 and 8 h. Results: There was good agreement for all chemistry and microscopy parameters with the exceptions of white blood cells (WBCs) at 24 and 72 h and red blood cells (RBCs) at 72 h. Preservative tubes on ice showed a significant decrease in concordance of WBCs and calcium oxalate (CaOx) parameters compared with the results at room temperature. Results of refrigerated polystyrene tubes showed good agreement with the exceptions of WBC clumps and amorphous crystal at 8 h. Conclusions: A chlorhexidine-containing preservative tube seems advantageous for urine sample transport from outside healthcare services. A preservative tube offers comparable results with urine samples kept in a refrigerator for 4-8 h for the majority of parameters. Keeping samples at room temperature is recommended when preservative tubes are used because ice produces a negative effect on WBCs and CaOx.en_US
dc.identifier.citationErcan, M., Akbulut, E. D., Abuşoğlu, S., Yılmaz, F. M., Oğuz, E. F., Topcuoğlu, C., Öztekin, V., Boğdaycıoğlu, N., (2015). Stability of Urine Specimens Stored with and Without Preservatives At Room Temperature and On Ice Prior to Urinalysis. Clinical Biochemistry, 48 (13-14), 919-922. DOI: 10.1016/j.clinbiochem.2015.05.016
dc.identifier.doi10.1016/j.clinbiochem.2015.05.016en_US
dc.identifier.endpage922en_US
dc.identifier.issn0009-9120en_US
dc.identifier.issn1873-2933en_US
dc.identifier.issue13-14en_US
dc.identifier.pmid26025772en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage919en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.clinbiochem.2015.05.016
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32572
dc.identifier.volume48en_US
dc.identifier.wosWOS:000361259700019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAbuşoğlu, Sedat
dc.language.isoenen_US
dc.publisherPergamon-Elsevıer Scıence Ltden_US
dc.relation.ispartofClinical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPreanalytic Phaseen_US
dc.subjectUrinalysisen_US
dc.subjectPreservative Tubeen_US
dc.titleStability of Urine Specimens Stored with and Without Preservatives At Room Temperature and On Ice Prior to Urinalysisen_US
dc.typeArticleen_US

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