Immunohistochemical and qPCR determination of the expression and serum level of anti-Mullerian hormone in pre-pubertal, intact and ovarian remnant syndrome detected bitches

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dc.contributor.authorAlkan, Kübra Karakaş
dc.contributor.authorCeylan, Ahmet
dc.contributor.authorAlkan, Hasan
dc.contributor.authorÖzen, Doğukan
dc.contributor.authorBayraktaroğlu, Alev Gürol
dc.contributor.authorKaymaz, Mustafa
dc.date.accessioned2020-03-26T20:14:35Z
dc.date.available2020-03-26T20:14:35Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractThe aim of this study was to determine the serum concentrations, ovarian presence and expression of anti-Mullerian hormone (AMH) in pre-pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre-pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (p < 0.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (p < 0.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre-pubertal groups, this increase was statistically non-significant (p > 0.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (p < 0.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (p < 0.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.en_US
dc.description.sponsorshipAnkara Universitesi [15B0239006]en_US
dc.description.sponsorshipAnkara Universitesi, Grant/Award Number: 15B0239006en_US
dc.identifier.citationKarakas Alkan, K., Ceylan, A., Alkan, H., Ozen, D., Bayraktaroglu, A. G., Kaymaz, M. (2019). Immunohistochemical And Qpcr Determination Of The Expression And Serum Level Of Anti‐Müllerian Hormone İn Pre‐Pubertal, İntact And Ovarian Remnant Syndrome Detected Bitches. Reproduction İn Domestic Animals, 54,(7), 979-986.
dc.identifier.doi10.1111/rda.13451en_US
dc.identifier.endpage986en_US
dc.identifier.issn0936-6768en_US
dc.identifier.issn1439-0531en_US
dc.identifier.issue7en_US
dc.identifier.pmid31050839en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage979en_US
dc.identifier.urihttps://dx.doi.org/10.1111/rda.13451
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37902
dc.identifier.volume54en_US
dc.identifier.wosWOS:000475980800006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAlkan, Hasan
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofREPRODUCTION IN DOMESTIC ANIMALSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAMHen_US
dc.subjectbitchen_US
dc.subjectimmunohistochemistryen_US
dc.subjectovarian remnant syndromeen_US
dc.subjectqPCRen_US
dc.titleImmunohistochemical and qPCR determination of the expression and serum level of anti-Mullerian hormone in pre-pubertal, intact and ovarian remnant syndrome detected bitchesen_US
dc.typeArticleen_US

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