Evaluation of Chromosome 8 and 11 Aneuploidies in Washings and Biopsy Materials of Bladder Transitional Cell Carcinoma
Yükleniyor...
Dosyalar
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
We compared chromosome 8 and 11 aneuploidies on bladder biopsy tumor tissues and bladder washing samples of transitional cell carcinoma (TCC) and their relationship to tumor malignancy. Interphase fluorescence in situ hybridization (FISH) was applied to nuclei of washing material and biopsy samples of 17 patients with TCC. Incidence of cells having aneuploidy was clearly nonrandom from patient to patient. There was no significant difference in the incidence of aneuploid frequency for chromosomes 8 and I I between biopsies of bladder tumors and bladder washing samples (P > 0.05). For chromosome 8, incidence of disomic cells (having two signals) in grade III tumors was significantly lower than in grade 11 tumors of both washing samples (P = 0.004) and biopsy materials (P = 0.005), indicating a high frequency of aneuploidy. The incidence of nuclei with four or more than four signals of chromosome 8 was significantly higher in grade III tumors than in grade 11 tumors in washing samples (P = 0.031 and 0.003, respectively). Similarly, in biopsy material, the incidence of nuclei with more than four signals of chromosome 8 was significantly higher in grade III tumors than in grade 11 tumors (P = 0.004). For chromosome 11, in both washing samples and biopsy materials, the incidence of disomic cells (having two signals) in grade III tumors was significantly lower than that detected in grade 11 tumors (P = 0.031 and 0.014, respectively), indicating a high frequency of aneuploidy. In biopsy materials, the incidence of nuclei with three or four signals was significantly higher than that in grade 11 tumors (P = 0.014 and 0.012, respectively). These findings suggest that FISH analysis of bladder washing samples can be efffectively detected as genetic changes of bladder tumors. It might predict genetic progression of these tumors, which might be related to tumor stage, because higher stages of tumors showed a higher incidence of aneuploidies of chromosomes 8 and 11. (C) 2003 Elsevier Science Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Kaynak
Cancer Genetics and Cytogenetics
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
142
Sayı
1
Künye
Acar, H., Kılınç, M., Yıldırım, M. S., Kaynak, M., Cenker, A., (2003). Evaluation of Chromosome 8 and 11 Aneuploidies in Washings and Biopsy Materials of Bladder Transitional Cell Carcinoma. Cancer Genetics and Cytogenetics, 142(1), 25-29. Doi: 10.1016/S0165-4608(02)00803-8
