Effects of cooling on histamine-induced contractions of human umbilical artery: The role of ion channels
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PROUS SCIENCE, SA
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of cooling (to 28 degrees C) on histamine (10(-9) - 3 x 10(-4) M)-induced contractions and the role of calcium (Ca2+), potassium (K-Ca(2+)) and sodium (Na+) channel blockers in the cooling-induced responses were investigated in the endothelium-denuded human umbilical artery. Concentration-response curves to histamine were isometrically recorded at 37 and 28 degrees C (control). The same procedure was repeated at 28 degrees C in the presence of tetraethylammonium (TEA, 10(-3) M), pilsicainide (10(-6) M), ouabain (10-6 M), caffeine (3 X 10-4 M), verapamil (10(-6) M) and also in Ca2+-free medium with ethylene glycol bis-(beta-aminoethyl ether) N,N,N-1,N-1-tetraacetic acid (EGTA). During cooling, the sensitivity, but not the maximal response, was significantly higher than 37 degrees C. Cooling to 28 degrees C after treatment with verapamil or pilsicainide decreased the sensitivity, whereas treatment with TEA and ouabain significantly increased sensitivity. Treatment with caffeine did not modify the effect of cooling. Furthermore, cooling to 28 degrees C after incubation in Ca(2+)free solution with EGTA decreased the sensitivity to histamine. The results of this study suggest the role of Ca2+, K-Ca(2+) and Na+-ion channels in the cooling-induced changes of human umbilical arteries treated with histamine. (C) 2007 Prous Science. All rights reserved.
Açıklama
Anahtar Kelimeler
cooling, histamine, human umbilical artery, Ca2+, K-Ca(2+) and Na+-ion channel blockers
Kaynak
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
29
Sayı
9
