Immunosensor Based on Fluorescence Quenching Matrix of the Conducting Polymer Polypyrrole
Yükleniyor...
Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Heidelberg
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the combination of autofluorescent proteins and fluorescence quenching polymers was shown to be a design which can increase the selectivity and sensitivity of immunosensors. With this objective, the conducting polymer polypyrrole (Ppy) was used as a matrix for immobilization of proteins, which enables biological recognition of the analyte, and as a fluorescence quencher, which increases the selectivity of fluorescence-based detection. In this study, bovine leukemia virus proteins gp51 were immobilized within the Ppy matrix and formed a polymeric layer with affinity for antibodies against protein gp51 (anti-gp51). The anti-gp51 antibodies are present at high levels in the blood serum of cattle infected by bovine leukemia virus. Secondary antibodies labeled with horseradish peroxidase (HRP) were used as specific fluorescent probes for detection of a particular target, because the fluorescence of HRP was readily detectable at the required sensitivity. The Ppy was used as fluorescent background, because its fluorescence was almost undetectable when excited by near UV light at 325 nm. Moreover the Ppy quenched the fluorescence of some fluorescent agents including fluorescein-5(6)-isothiocyanate (fluorescein), rhodamine B, and HRP by almost 100% when these fluorescent agents were adsorbed on the surface of Ppy. It is predicted that Ppy-induced fluorescence quenching could be used in the design of immunosensors to increase selectivity and sensitivity.
Açıklama
43rd Annual Meeting of the German-Society-for-Mass-Spectrometry -- MAR 07-10, 2010 -- Halle/Saale, GERMANY
Anahtar Kelimeler
Immunosensor, Fluorescence quenching, Conducting polymers, Polypyrrole, Nanotechnology
Kaynak
Analytical and Bioanalytical Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
398
Sayı
Künye
Ramanavicius, A., Ryskevic, N., Öztekin, Y., Kausaite-Minkstimiene, A., Jursenas, S., Baniukevic, J., Kirlyte, J., Bubniene, U., Ramanaviciene, A., (2010). Immunosensor Based on Fluorescence Quenching Matrix of the Conducting Polymer Polypyrrole. Analytical and Bioanalytical Chemistry, (398), 3105-3113. Doi: 10.1007/s00216-010-4265-8
