Immunosensor Based on Fluorescence Quenching Matrix of the Conducting Polymer Polypyrrole

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dc.contributor.authorRamanavicius, A.
dc.contributor.authorRyskevic, N.
dc.contributor.authorÖztekin, Y.
dc.contributor.authorKausaite-Minkstimiene, A.
dc.contributor.authorJursenas, S.
dc.contributor.authorBaniukevic, J.
dc.contributor.authorKirlyte, J.
dc.contributor.authorBubniene, U.
dc.contributor.authorRamanaviciene, A.
dc.date.accessioned2020-03-26T17:49:05Z
dc.date.available2020-03-26T17:49:05Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description43rd Annual Meeting of the German-Society-for-Mass-Spectrometry -- MAR 07-10, 2010 -- Halle/Saale, GERMANYen_US
dc.description.abstractIn this study, the combination of autofluorescent proteins and fluorescence quenching polymers was shown to be a design which can increase the selectivity and sensitivity of immunosensors. With this objective, the conducting polymer polypyrrole (Ppy) was used as a matrix for immobilization of proteins, which enables biological recognition of the analyte, and as a fluorescence quencher, which increases the selectivity of fluorescence-based detection. In this study, bovine leukemia virus proteins gp51 were immobilized within the Ppy matrix and formed a polymeric layer with affinity for antibodies against protein gp51 (anti-gp51). The anti-gp51 antibodies are present at high levels in the blood serum of cattle infected by bovine leukemia virus. Secondary antibodies labeled with horseradish peroxidase (HRP) were used as specific fluorescent probes for detection of a particular target, because the fluorescence of HRP was readily detectable at the required sensitivity. The Ppy was used as fluorescent background, because its fluorescence was almost undetectable when excited by near UV light at 325 nm. Moreover the Ppy quenched the fluorescence of some fluorescent agents including fluorescein-5(6)-isothiocyanate (fluorescein), rhodamine B, and HRP by almost 100% when these fluorescent agents were adsorbed on the surface of Ppy. It is predicted that Ppy-induced fluorescence quenching could be used in the design of immunosensors to increase selectivity and sensitivity.en_US
dc.description.sponsorshipGerman Soc Mass Spectrometryen_US
dc.identifier.citationRamanavicius, A., Ryskevic, N., Öztekin, Y., Kausaite-Minkstimiene, A., Jursenas, S., Baniukevic, J., Kirlyte, J., Bubniene, U., Ramanaviciene, A., (2010). Immunosensor Based on Fluorescence Quenching Matrix of the Conducting Polymer Polypyrrole. Analytical and Bioanalytical Chemistry, (398), 3105-3113. Doi: 10.1007/s00216-010-4265-8
dc.identifier.doi10.1007/s00216-010-4265-8en_US
dc.identifier.endpage3113en_US
dc.identifier.issn1618-2642en_US
dc.identifier.issn1618-2650en_US
dc.identifier.pmid20941482en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage3105en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00216-010-4265-8
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24988
dc.identifier.volume398en_US
dc.identifier.wosWOS:000284542300038en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖztekin, Y.
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectImmunosensoren_US
dc.subjectFluorescence quenchingen_US
dc.subjectConducting polymersen_US
dc.subjectPolypyrroleen_US
dc.subjectNanotechnologyen_US
dc.titleImmunosensor Based on Fluorescence Quenching Matrix of the Conducting Polymer Polypyrroleen_US
dc.typeArticleen_US

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