Isolation and culture of adult mouse vestibular nucleus neurons

Basit Öğe Kaydı
dc.contributor.authorHim, Aydın
dc.contributor.authorAltuntaş, Serap
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorErdoğan, Ender
dc.contributor.authorCengiz, Nurettin
dc.date.accessioned2020-03-26T19:41:58Z
dc.date.available2020-03-26T19:41:58Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground/aim: Isolated cell cultures are widely used to study neuronal properties due to their advantages. Although embryonic animals are preferred for culturing, their morphological or electrophysiological properties may not reflect adult neurons, which may be important in neurodegenerative diseases. This paper aims to develop a method for preparing isolated cell cultures of medial vestibular nucleus (MVN) from adult mice and describe its morphological and electrophysiological properties. Materials and methods: Vestibular nucleus neurons were mechanically and enzymatically isolated and cultured using a defined medium with known growth factors. Cell survival was measured with propidium iodide, and electrophysiological properties were investigated with current-clamp recording. Results: Vestibular neurons grew neurites in cultures, gaining adult-like morphological properties, and stayed viable for 3 days in culture. Adding bovine calf serum, nerve growth factor, or insulin-like growth factor into the culture medium enhanced neuronal viability. Current-clamp recording of the cultured neurons revealed tonic and phasic-type neurons with similar input resistance, resting membrane potential, action potential amplitude, and duration. Conclusion: Vestibular neurons from adult mice can be cultured, and regenerate axons in a medium containing appropriate growth factors. Culturing adult vestibular neurons provides a new method to study age-related pathologies of the vestibular system.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG2971-104S506]en_US
dc.description.sponsorshipThis work was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK, Grant No. SBAG2971-104S506).en_US
dc.identifier.doi10.3906/sag-1706-158en_US
dc.identifier.endpage1911en_US
dc.identifier.issn1300-0144en_US
dc.identifier.issn1303-6165en_US
dc.identifier.issue6en_US
dc.identifier.pmid29306256en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1903en_US
dc.identifier.urihttps://dx.doi.org/10.3906/sag-1706-158
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35242
dc.identifier.volume47en_US
dc.identifier.wosWOS:000418884300035en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF MEDICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectVestibular nucleusen_US
dc.subjectprimary cultureen_US
dc.subjectregenerationen_US
dc.subjectmouseen_US
dc.subjectwhole-cell recordingen_US
dc.titleIsolation and culture of adult mouse vestibular nucleus neuronsen_US
dc.typeArticleen_US

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