Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c
Yükleniyor...
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE BV
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This work presents the immobilization of trypsin on the synthesized pillar[5]arene derivative (P5) containing ten iodo-terminals. The characterization has been carried out by Thermogravimetric Analysis (TGA), Fourier Transform Infrared (FTIR) and Raman spectra, Scanning Electron Microscopy (SEM) and Zeta Potential. Furthermore, Cytochrome c (Cyt c) was chosen as a model protein for evaluation of the performance of the pillar [5]arene-immobilized trypsin (P5-T), and its microwave-assisted digestion conditions were investigated by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for 15 second. The peptide numbers of 10 and sequence coverage of 93% for 15 second microwave-assisted digestion were obtained for P5-T. The results can be further applied in future proteomics studies due to high efficiency, good reproducibility and stability of the pillar[5]arene-immobilized trypsin.
Açıklama
Anahtar Kelimeler
Pillar[5]arene, Trypsin, Immobilization, Cytochrome c, Microwave digestion
Kaynak
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
94
Sayı
Künye
Atacan, K., Kursunlu, A. N., Ozmen, M. (2019). Preparation Of Pillar [5] Arene İmmobilized Trypsin And İts Application İn Microwave-Assisted Digestion Of Cytochrome C. Materials Science And Engineering: C, 94, 886-893.
